Dissociation curves from KRAS analytical sensitivity experiment for G12D KRAS mutation. The analysis corresponds to 5%, 2.5%, 1%, 0.5%, and 0% G12D in different amounts of DNA background from T-84 cell line: for background DNA of 70 ng/reaction (a) and 35 ng/reaction (b) the PCR products of 5%, 2.5%, 1%, 0.5% mutated DNA show specific melting curve until 0.5%. For background DNA of 5 ng/reaction (c) the specific dissociation curve is present until 1%.