Research Article

Neutrophil Inhibitory Factor Selectively Inhibits the Endothelium-Driven Transmigration of Eosinophils In Vitro and Airway Eosinophilia in OVA-Induced Allergic Lung Inflammation

Table 1

Cumulative effects of blocking mAbs in the presence of rNIF on transendothelial migration of eosinophils across IL-4-activated HUVEC monolayers.

AntigenInhibition of eosinophil transendothelial migration (%)
mAbBuffer rNIF 10 nM
Mean SDMean SD

HLA I chainW6/329.10.775.20.9
CD18CLB5471.31.9100.40.4
VLA-4HP2/141.72.285.70.9
CD11a/CD1825.348.71.076.51.2
CD11b/CD1860.171.30.967.81.2
CD11b A-domain MIDAS10765.21.066.11.0

Eosinophils were either incubated with the indicated mAbs alone or together with 10 nM rNIF. Data are given as mean of triplicate determinations ±SD.