Combination Phototherapy with a Histone Deacetylase Inhibitor and a Potent DNA-Binding Bibenzimidazole: Effects in Haematological Cell Lines
Figure 2
SAHA induces the accumulation of hyperacetylated histone and nonhistone protein in malignant and transformed haematological cells. Chemical structure of SAHA (a). Effects of SAHA on histone H2B, H3, and H4 acetylation, as well as α-tubulin acetylation in haematological K562, CEM-CCRF, R100, and LCL cells. Cells were pretreated with 10 μM of SAHA for 24 hours prior to extraction of whole cell lysates. Immunoblots of specific antibodies to acetylated histones H2B, H3, H4, and α-tubulin are shown. Equal loading and transfer were verified by probing the membranes with GAPDH (b).
