Research Article
Selectivity of Brij-35 in Micellar Liquid Chromatographic Separation of Positional Isomers
Table 1
Retention time and resolution data for various positional isomers using hydro-organic and hybrid surfactant mobile phase composition.
| | Analyte | Solvent composition |
*
(R) | Solvent composition |
*
(R) |
| | 2-nitroaniline | | 2.87 | 88 : 12 | 4.57 | | 3-nitroaniline | 1 : 1 MeOH/water | 2.28 (1.99) | Brij35 (2% aq. soln.)/propanol | 3.7 (2.56) | | 4-nitroaniline | | 1.99 (1.14) | 3.43 (0.92) |
| | 2-quinolinol | 1 : 1MeOH/water | 2.2 | 99 : 1 | 2.53 | | 4-quinolinol | 1.67 (1.53) | Brij-35 (1.5% aq. soln.)/propanol | 1.78 (1.629) |
| | o-cresol | | 2.03 | 90 : 10 | 1.69 | | p-cresol | 7 : 3 MeOH/water | 1.96 | Brij35 (1.75% aq. soln.)/propanol | 1.65 | | m-cresol | | 1.96 | 1.65 |
| | m-nitrophenol | 7 : 3 MeOH/water | 2.27 | 88 : 12 | 1.83 | | p-nitrophenol | 2.07 (0.453) | Brij-35 (2% aq. soln.)/propanol | 1.62 (1.35) |
| | Dexamethazone | 7 : 3 MeOH/water | 2.49 | 99 : 1 | 4.24 | | Betamethasone | 2.52 | Brij-35 (1.5% aq. soln.)/propanol | 4.04 |
| | Propylparaben | 7 : 3 MeOH/water | 2.3 | 96 : 4 | 7.33 | | iso-propylparaben | | 2.21 (0.32) | Brij-35 (1.75% aq. soln.)/propanol | 6.47 (0.809) |
| | Bezoic acid | 4 : 6 MeOH/water | 1.78 | 90 : 10 | 4.92 | | Sorbic acid | 2.24 (0.64) | Brij-35 (1.5% aq. soln.)/propanol | 3.88 (1.73) |
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*
is retention time in minutes; in parenthesis “R” is resolution.
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