International Journal of Carbohydrate Chemistry

Research Article

Partial Chemical Characterization of Immunomodulatory Polysaccharides from Plantago palmata Hook. f. s. Leaves

Table 1

Molecular weights of polymers obtained from Plantago palmata leaves by aqueous extraction (50°C, 3 hours), concentration and extensive dialysis (PS50) and further filtration (PS50F) on a Millipore cellulose filter (0.22 m). SEC analysis referring to a calibration curve obtained with pullulan standards (n = 3).


Polymer	Elution volume					Polydispersity
Polymer	(mL)	(g/mol)	(g/mol)	(g/mol)	(g/mol)	(/)

	11.8 to 13.8 mL^a	1129867 (39000)	1192500 (51000)	1255767 (65000)	1190167 (37000)	1.05
PS50	13.8 to 16.8 mL^b	222537 (16000)	271913 (12000)	322567 (8000)	303167 (12000)	1.22
	16.8 to 19.6 mL^c	39485 (650)	11818 (720)	14856 (760)	9895 (550)	1.24

	11.8 to 13.8 mL	1189650 (9000)	1278550 (14000)	1369650 (20000)	1262000 (13000)	1.07
PS50F	13.8 to 16.8 mL	230515 (1000)	277390 (620)	325485 (605)	304600 (6000)	1.20
	16.8 to 19.6 mL	10338 (250)	12860 (720)	16068 (960)	10597 (3300)	1.24

= Number average molecular weight; = Weight average molecular weight; = Z average molecular weight; = Peak molecular weight.
^aCorresponds to fraction F2-1 (Figure 1).
^bCorresponds to fraction F2-2 plus part of F2-3 enclosing the tail of the major peak.
^cComprehends the peak skimmed from the major peak (corresponds to the end of fraction F2-3 plus part of F3-1).