Figure 4: Effect of temperature gradient on spermine-induced root abscission in A. pinnata. Root abscission is represented as % of the initial number of roots following addition of 2 mM spermine at time zero. The assays were carried out at 25°C and 38°C ( 𝑛 = 6 ) and 27°C and 32°C ( 𝑛 = 9 ). Standard error bars are only shown where larger than the symbols used. Assays were conducted in 10 mM potassium phosphate solution (pH 7) at the time zero.