Research Article
Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts
Table 2
Accuracy results of MLC method: analysis of sesquiterpenic acid mixtures and standard addition technique.
| Compound | Added (μg·mL−1) | As found*(μg·mL−1) | % Recovery | | Added, μg·mL−1 | As found*, μg·mL−1 | Recovery, % | with addition | without addition |
| Hydroxyvalerenic acid | 1.90 | 1.88 | 99.0 | −1.0 | 0 | — | 2.10 | | 9.30 | 9.25 | 99.5 | −0.5 | 1.90 | 4.20 | 2.20 | 104.8 | 27.9 | 27.8 | 99.6 | −0.4 | 5.60 | 7.74 | 2.14 | 102.1 | | | | | 9.3 | 11.4 | 2.13 | 101.7 | Mean, %R.S.D. | 99.4, 0.8 | | | | Mean, % | 102.9 |
| Acetoxyvalerenic acid | 4.20 | 4.19 | 99.8 | −0.2 | 0 | — | 33.4 | | 21.0 | 20.85 | 99.3 | −0.7 | 12.6 | 46.3 | 33.7 | 102.1 | 63.0 | 63.1 | 100.1 | +0.1 | 21.0 | 54.2 | 33.2 | 99.4 | | | | | 29.4 | 63.5 | 34.1 | 102.1 | Mean, %R.S.D. | 99.7, 1.1 | | | | Mean, % | 101.2 |
| Valerenic acid | 6.10 | 6.07 | 99.5 | −0.5 | 0 | — | 50.7 | | 30.4 | 30.5 | 100.3 | +0.3 | 18.2 | 69.4 | 51.2 | 101.0 | 91.2 | 91.4 | 100.2 | +0.2 | 30.4 | 80.7 | 50.3 | 99.2 | | | | | 42.6 | 93.6 | 51.0 | 100.6 | Mean, %R.S.D. | 100.0, 1.1 | | | | Mean, % | 100.3 |
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*Mean value of the four determinations.
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