Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts
Table 3
Results from sesquiterpenic acids assay in various samples using RP-HPLC and MLC methods and verification of the variance of significance between two methods.
MLC*
HPLC*
Δ
Calculated F values**
HVal, %
AcVal, %
Val, %
Sum, %
HVal, %
AcVal, %
Val, %
Sum, %
HVal, %
AcVal, %
Val, %
Sum, %
HVal
AcVal
Val
Valerian root and rhizome (3 samples)
0.005
0.073
0.088
0.166
0.006
0.070
0.087
0.163
−0.001
−0.003
+0.001
+0.003
3.152
4.261
2.154
0.001
0.071
0.084
0.156
0.001
0.069
0.082
0.152
0
+0.002
+0.002
+0.004
1.555
3.644
2.233
0.002
0.082
0.098
0.182
0.001
0.085
0.099
0.185
+0.001
−0.003
−0.001
−0.003
4.176
3.954
3.771
Valerian dry extract (3 samples)
0.031
0.142
0.178
0.351
0.033
0.145
0.177
0.355
−0.002
−0.003
+0.001
−0.004
3.244
1.547
3.334
0.045
0.133
0.201
0.379
0.047
0.132
0.204
0.383
+0.002
+0.001
−0.003
−0.004
3.672
3.674
1.780
0.028
0.186
0.214
0.428
0.030
0.188
0.213
0.431
−0.002
−0.002
+0.001
−0.003
2.966
2.425
2.655
HVal: hydroxyvalerenic acid, AcVal: acetoxyvalerenic acid, Val: valerenic acid, and Sum: total contents of valerenic acids.
*Mean value of the four determinations
**Tabulated F value is 6.38.
