Chromatin-Associated Proteins Revealed by SILAC-Proteomic Analysis Exhibit a High Likelihood of Requirement for Growth Fitness under DNA Damage Stress

<table>Abundant ribosomal proteins are effectively depleted in CHRAP-prep by SILAC. (a) A schematic diagram shows the steps of CHRAP preparation. WCE stands for whole cell extract; sup for supernatant; pel for pellet; and resusp. for resuspension. (b) The image of the agarose gel shows the presence or absence of genomic DNA (gDNA) in various samples indicated as in (a). (c) The image of western blot shows the presence or absence of histone H4 and soluble protein tubulin (tub). (d) Top 10% ranked proteins by SILAC ratios are depleted of ribosomal proteins. <i>X</i>-axis indicates the percentile ranks by SILAC ratios and <i>Y</i>-axis indicates the level of the ribosomal proteins.</table>

International Journal of Proteomics

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Figure 3

Figure 3: Chromatin-Associated Proteins Revealed by SILAC-Proteomic Analysis Exhibit a High Likelihood of Requirement for Growth Fitness under DNA Damage Stress 

Figure 3 | Chromatin-Associated Proteins Revealed by SILAC-Proteomic Analysis Exhibit a High Likelihood of Requirement for Growth Fitness under DNA Damage Stress 