Traditional CHRAP assay to validate SILAC-enriched/depleted proteins. (a) SILAC-enriched nucleus localized proteins are association with the chromatin. Loading quantity of various protein samples such as whole cell extract (W), supernatant-1 (s1) and supernatant-2 (s2; the CHRAP-prep) is indicated in parentheses on the top. Tested proteins and their SILAC ratio in parentheses are indicated at the bottom. (b) SILAC-depleted proteins are unassociated with the chromatin. The display is identical to (a). (c) Subcellular localization of the HA-tagged Srp2 proteins. (d) SILAC-enriched dual-localized proteins are associated with the chromatin. The display is identical to (a).