Research Article

Differences in Chemical Sexual Signals May Promote Reproductive Isolation and Cryptic Speciation between Iberian Wall Lizard Populations

Table 2

Lipophilic compounds found in femoral gland secretions of male lizards, P. hispanica, from five distinct populations of the Madrid region (Aranjuez, Belmonte, Golondrina, Fuenfría, and Pedrezuela). The relative amount of each component was determined as the percent of the total ion current (TIC) and reported as the average (±SE). Characteristics ( ) are reported for some unidentified (Un.) compounds. (RT: retention time).

CompoundsRT (min)FuenfríaPedrezuelaGolondrinaBelmonteAranjuez

Steroids
Un. steroid
(145,213,248,353,368,387)
29.92
Cholesta-2-4-diene30.58
Cholesta-3.5-diene30.81
Un. steroid (155,197,251,350,365)30.96 0.55 ± 0
Cholesta-5,7,9(11)-trien-3-ol31.06
Un. steroid (207,251,350,365)31.13
Un. steroid (143,195,207,351,366)31.20
Un. steroid (141,156,209,350,365)31.37
Un. steroid (155,197,251,365,379)31.64
Un. steroid (195,209,251,365,379)31.84
Cholesterol32.43
Cholestanol32.47
Cholesta-5.7-dien-3-ol.32.65
Un.steroid (105,213,255,353,368,386,415)32.75
Ergosterol33.00
Campesterol33.17
Cholest-4-en-3-one33.41
Ergosta-5,8-dien-3-ol33.50
Cholesta-4,6-dien-3-one33.69
Sitosterol33.92
Ergostanol34.02
Stigmasterol34.13
Un.steroid (221,253,281,355,380,430)34.30
Cholest-5-en-3-one34.38
Ergosta-5.22-dien-3-ol34.47
Un.steroid (214,267,395)35.30

Carboxylic acids and their esters
Tetradecanoic acid20.64
Pentadecanoic acid21.68
Hexadecanoic acid. methyl ester22.33
Hexadecenoic acid22.54
Hexadecanoic acid22.76
Hexadecanoic acid, ethyl ester22.98
9,12-octadecadienoic acid24.35
Octadecenoic acid24.43
Octadecanoic acid24.60
Octadecanoic acid, ethyl ester24.82
Eicosanoic Acid26.31
Docosanoic acid28.00
Docosanoic acid, ethyl ester28.21

Alcohols
Hexadecanol21.02
Octadecanol23.87
Eicosanol25.67
Docosanol27.33
Tetracosanol29.80

Waxy esters
Unidentified waxy ester 129.45
Unidentified waxy ester 235.57
Unidentified waxy ester 338.06
Unidentified waxy ester 438.27

Others
Tetradecanone22.11
Unidentified Furanone24.19
Squalene30.07
Unidentified terpenoid 130.83
Unidentified terpenoid 231.94