|Table 1: Summary of results of cycle sequencing purification methods examined in this study.|
|Sequence length and quality scores for overlapping fragments were obtained directly from Sequencher 4.8 software (Gene Codes Corporation, Inc., Ann Arbor, MI). Prior to analyzing the data, raw sequences were trimmed by aligning all of the sequences of one reaction and determining the unusable part of the longest sequence and cutting all sequences at that base. Measurements and standard deviation are calculated using triplicate results of 4 different reactions (primers used were con2, con3, VP7R and 9con1L). Measurement based on *1 and **2 fewer sequences due to low quality of final sequence. |
Cost per reaction was calculated by totaling the cost of consumables needed to clean one 10 L cycle sequencing reaction and does not account for the cost of equipment.
Time needed to purify 96 samples was measured from the moment the plate containing the cycle sequencing reactions was removed from the thermocycler after cycle sequencing to the time purification was completed and the 96-well septum was placed on the plate.