Table 2
(a)

Trial Number Mobile phase AComposition ratio of mobile phase A

1Acetonitrile : Water : Formic acid 500 : 500 : 1
2Acetonitrile : Water : Formic acid300 : 700 : 1
3Acetonitrile : Water : Formic acid250 : 750 : 1
4Acetonitrile : Water : Formic acid285 : 715 : 1
5Acetonitrile : Water : Formic acid285 : 715 : 1

Mobile phase A used for different trials.
(b)

Trial NumberMobile phase BComposition ratio of mobile phase B

1Acetonitrile : Water : Formic acid500 : 500 : 1
2Acetonitrile : Water : Formic acid800 : 200 : 1
3Acetonitrile : Water : Formic acid800 : 200 : 1
4Acetonitrile : Water : Formic acid800 : 200 : 1
5Methanol : Water : Formic acid800 : 200 : 1

Mobile phase B used for different trials.