International Journal of Evolutionary Biology

Research Article

Analysis of the Meiotic Segregation in Intergeneric Hybrids of Tilapias

Table 2

Microsatellite loci analysed with indications of GenBank accession number, repeat structure, and linkage group according to O. niloticus [47, 48, 51], optimised PCR conditions established from cross-species amplification study of microsatellites across 15 African cichlid species (labeled primer “*”, annealing temperature and Magnesium concentration (mM)—Bezault et al., [52]); the size range and diversity of alleles within each parental species as well as the number of shared alleles between them and the presence of null allele (N) have been estimated from the set of individuals used for cross-priming analysis and the parents of all families studied here; the set of independent loci analysed in all backcross and F2 hybrid families are indicated in bold.


Loci	GenBank accession	Structure	Linkage group	PCR conditions	Range (bp)	Allelic diversity		Shared alleles
Loci	GenBank accession	Structure	Linkage group	PCR conditions	Range (bp)	O. niloticus	S. melanotheron	Shared alleles

UNH-008	G31346	perfect	17	R* 56/1.2	196–236	3	2	0
UNH-102	G12255	perfect	16	R* 50/1.2	132–185	4	4	0
UNH-103	G12256	perfect	17	R* 48/1.2	171–260	3	4	0
UNH-106	G12259	compound	3	R* 50/1.2	115–189	4	2 + N	1
UNH-115	G12268	compound	3	F* 50/1.5	100–146	3	1	0
UNH-117	G12270	interrupted		R* 54/1.2	108–146	1	2	1
UNH-123	G12276	perfect	12	F* 48/1.2	142–232	6	2	0
UNH-124	G12277	perfect	4	F* 54/1.2	295–324	4	1	0
UNH-125	G12278	compound	16	R* 48/1.5	134–198	6	4	2
UNH-129	G12282	interrupted	8	R* 48/1.2	180–253	7	4	1
UNH-130	G12283	perfect	23	R* 50/1.2	174–242	6	1 + N	0
UNH-131	G12284	perfect	3	F* 48/2.0	283–303	4	2 + N	0
UNH-132	G12285	perfect	9	R* 52/1.2	100–134	2	1 + N	0
UNH-135	G12287	interrupted	3	R* 50/1.5	124–284	6	4	1
UNH-138	G12290	perfect	16	R* 48/1.5	144–250	7	2	0
UNH-142	G12294	interrupted		F* 48/1.2	142–192	3	2	0
UNH-146	G12298	interrupted	4	F* 60/1.0	111–149	3	3	1
UNH-149	G12301	perfect	5	R* 48/1.5	143–225	4	3	0
UNH-154	G12306	perfect	6	R* 50/1.2	98–176	8	5	2
UNH-159	G12311	perfect	2	R* 55/1.2	205–267	5 + N	3	1
UNH-162	G12314	perfect	4	R* 48/1.5	125–252	6	2	0
UNH-169	G12321	interrupted	5	R* 54/1.2	124–240	8	4 + N	1
UNH-173	G12325	perfect	13	F* 55/1.2	124–188	2	1 + N	0
UNH-174	G12326	perfect	20	F* 48/1.5	146–187	4	1	0
UNH-189	G12341	perfect	12	R* 52/1.2	135–208	3	3	1
UNH-190	G12342	compound	21	R* 60/1.0	133–202	2	1	0
UNH-197	G12348	interrupted	23	R* 50/1.2	154–228	6	5	0
UNH-207	G12358	interrupted	6	R* 60/1.2	90–198	3	4	1
UNH-211	G12362	perfect	19	R* 48/1.5	82–194	6	7	1
UNH-216	G12367	perfect	23	R* 52/1.2	126–212	2	4	0

				Average across loci		4.3	2.8	0.48
				Polymorphism P (0.95)		97%	77%