Sample 2D gel separation of whole-embryo proteins. Whole-embryos were solubilized and the proteins separated using IPG strips in the 1st dimension and SDS gradient slab gels in the 2nd dimension. The protein profiles were visualized by Coomassie blue staining. Nine MS/MS identified protein spots with differential expression due to alcohol treatment are highlighted with a white “x” and labeled with the gene name. The remaining spots with altered expression are labeled with the spot number in black font followed by “R,” signifying this gel as the reference (R) control.