Journal of Atomic, Molecular, and Optical Physics
Volume 2012 (2012), Article ID 948390, 5 pages
Silicon-Based Technology for Ligand-Receptor Molecular Identification
1Department of Biochemistry and Medical Biotechnologies, University of Naples “Federico II”, 80131 Naples, Italy
2Institute for Microelectronics and Microsystems, Unit of Naples, National Council of Research, 80131 Naples, Italy
3Department of Clinical and Experimental Medicine, University of “Magna Graecia”, 88100 Catanzaro, Italy
4CEINGE Advanced Biotechnology s.c.a.r.l, 80145 Naples, Italy
Received 23 June 2011; Accepted 4 October 2011
Academic Editor: Ali Hussain Reshak
Copyright © 2012 Annalisa Lamberti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
One of the most important goals in the fields of biology and medicine is the possibility to dispose of efficient tools for the characterization of the extraordinary complexity of ligand-receptor interactions. To approach this theme, we explored the use of crystalline silicon (cSi) technology for the realization of a biotechnological device in which the ligand-receptor interactions are revealed by means of optical measurements. Here, we describe a chemical procedure for the functionalization of microwell etched on silicon wafers, and the subsequent anchoring of biological molecules like an antibody anti-A20 murine lymphoma cell line. The optical analysis of the interaction on the biochips between the bound biomolecule and their corresponding ligand indicated that the functionalized cSi is suitable for this application.