Biochemical and Pharmacological Characterization of TLBbar, a New Serine Protease with Coagulant Activity from <i>Bothrops barnetti</i> Snake Venom

<table>Kinetic properties and inhibition of the thrombin-like enzyme TLBbar from <i>B. barnetti</i> venom upon BApNA substrate. (a) Proteolytic activity of the whole venom <i>B. barnetti</i> (WV) Bbt Ia and TLBbar fractions. (b) Inhibition of the proteolytic activity of TLBbar by PMSF (phenylmethyl sulfonyl fluoride), Leupeptin (acetyl-leucyl-leucyl-argial) EGTA (acid glycol-bis (2 aminoetileter)-N,N,N,N-tetracetic), EDTA (Ethylenediamine tetraacetic acid), SBT-I (Soybean trypsin inhibitor), and heparin. (c) Effect of substrate concentration. (d) Lineweaver-Burk (double-reciprocal) plot. (e) Effect of temperature. (f) Effect of pH. The results of all experiments are the <svg height="11.8" id="M27" style="vertical-align:-0.3003pt" version="1.1" viewbox="0 0 77.037498 11.8" width="77.037498" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>). </table>

Journal of Toxins

fig4

Figure 4

Figure 4: Biochemical and Pharmacological Characterization of TLBbar, a New Serine Protease with Coagulant Activity from <i>Bothrops barnetti</i> Snake Venom 

Figure 4 | Biochemical and Pharmacological Characterization of TLBbar, a New Serine Protease with Coagulant Activity from Bothrops barnetti Snake Venom 