Dataset Papers in Science

Dataset Paper

Alterations of Hormone-Sensitive Adenylyl Cyclase System in the Tissues of Rats with Long-Term Streptozotocin Diabetes and the Influence of Intranasal Insulin

Table 4

The stimulating effects of hormones on AC activity in (a) the heart, (b) brain, and (c) testes of diabetic and control rats with and without therapy by I-I. C: control animals; CI: control animals treated with intranasal insulin (I-I); D: diabetic animals with 210-day streptozotocin-induced diabetes mellitus of the type 1 (T1DM); DI: diabetic animals treated with I-I. Isoproterenol, noradrenaline, serotonin, EMD-386088, and dopamine were taken at 10⁻⁵ M, PACAP-38 at 10⁻⁷ M, and hCG at 10⁻⁸ M. Values are the weighted mean ± weighted standard deviation of three individual experiments, each performed in triplicate.

(a) In picomole (pmol) of cAMP/min per milligram (mg) of membrane protein


Groups of Rats	Isoproterenol	Noradrenaline

Group C (n = 8)	65.0 ± 2.7 (C-D, P = 0.0004; C-CI, P = 0.1348)	47.2 ± 1.1 (C-D, P = 10⁻⁸; C-CI, P = 0.4434)
Average of the first series	73.7	53.6
Average of the second series	54.9	48.1
Average of the third series	66.6	46.0
Group CI (n = 7)	60.3 ± 0.9 (CI-DI, P = 0.2191)	52.7 ± 1.9 (CI-DI, P = 0.1188)
Average of the first series	61.6	42.6
Average of the second series	61.1	56.7
Average of the third series	57.3	55.0
Group D (n = 8)	47.0 ± 1.8 (D-DI, P = 0.0032)	29.5 ± 1.6 (D-DI, P = 0.0025)
Average of the first series	43.9	30.3
Average of the second series	44.4	32.3
Average of the third series	55.2	25.8
Group DI (n = 7)	56.2 ± 1.7	37.9 ± 2.3
Average of the first series	53.8	31.9
Average of the second series	56.1	51.4
Average of the third series	62.0	49.8

(b) In picomole (pmol) of cAMP/min per milligram (mg) of membrane protein


Groups of Rats	Isoproterenol	Serotonin	EMD-386088	Dopamine	PACAP-38

Group C (n = 8)	42.6 ± 1.1 (C-D, P = 10⁻⁹; C-CI, P = 0.0374)	81.3 ± 2.2 (C-D, P = 0.0066; C-CI, P = 0.7467)	28.3 ± 1.8 (C-D, P = 0.0004; C-CI, P = 0.3930)	52.2 ± 1.0 (C-D, P = 0.1771; C-CI, P = 0.0050)	34.1 ± 0.3 (C-D, P = 10⁻⁸; C-CI, P = 0.0382)
Average of the first series	41.6	90.0	29.5	51.8	33.7
Average of the second series	42.6	81.1	27.3	50.0	36.3
Average of the third series	45.8	75.9	33.5	55.1	36.1
Group CI (n = 7)	40.3 ± 1.2 (CI-DI, P = 0.0226)	81.8 ± 2.1 (CI-DI, P = 0.0107)	34.2 ± 2.0 (CI-DI, P = 0.2647)	47.7 ± 1.2 (CI-DI, P = 0.4680)	37.8 ± 1.2 (CI-DI, P = 0.2633)
Average of the first series	40.6	83.2	30.0	46.6	40.6
Average of the second series	42.8	92.2	28.1	45.1	35.3
Average of the third series	37.3	75.1	39.0	51.2	38.7
Group D (n = 8)	28.0 ± 0.8 (D-DI, P = 0.0031)	67,2 ± 4.0 (D-DI, P = 0.1032)	21.4 ± 1.2 (D-DI, P = 0.00065)	44.4 ± 5.1 (D-DI, P = 0.8898)	22.3 ± 0.7 (D-DI, P = 10⁻⁵)
Average of the first series	26.8	51.5	20.5	56.8	22.2
Average of the second series	29.4	72.6	19.4	32.7	25.0
Average of the third series	30.0	75.3	23.4	47.6	19.2
Group DI (n = 7)	33.9 ± 1.7	74.8 ± 1.3	29.5 ± 2.0	46.2 ± 1.7	37.9 ± 2.0
Average of the first series	33.9	75.9	25.4	50.2	34.7
Average of the second series	40.5	76.7	29.6	43.3	32.8
Average of the third series	31.7	70.8	33.2	45.6	40.2

(c) In picomole (pmol) of cAMP/min per milligram (mg) of membrane protein


Groups of Rats	hCG	PACAP-38

Group C (n = 8)	105 ± 4 (C-D, P = 10⁻⁷; C-CI, P = 0.0270)	53 ± 3 (C-D, P = 10⁻⁵; C-CI, P = 0.4720)
Average of the first series	127	44
Average of the second series	105	52
Average of the third series	95	58
Group CI (n = 7)	89 ± 5 (CI-DI, P = 0.0039)	50 ± 2 (CI-DI, P = 0.0007)
Average of the first series	110	50
Average of the second series	76	45
Average of the third series	84	69
Group D (n = 8)	40 ± 3 (D-DI, P = 10⁻⁷)	29 ± 2 (D-DI, P = 0.1024)
Average of the first series	40	25
Average of the second series	47	35
Average of the third series	36	30
Group DI (n = 7)	67 ± 2	34 ± 2
Average of the first series	66	29
Average of the second series	63	36
Average of the third series	71	40