Research Article

Purification and Characterization of a Nonenzymatic Neurotoxin from Hippasa partita (Lycosidae) Spider Venom Gland Extract

Table 1

Summary of purification of neurotoxin from H. partita venom. Values are average of three independent experiments.

ProcedureTotal protein (mg)Protein recovery (%)Activity recovery (%)

Venom gland extract100100100
Sephadex G-100 column (peak IV)303082
CM-Sephadex C-25 column (peak II)62074
Purified neurotoxin (considering the protein loaded on first step, after desalting and lyophilizing)5574

Values are average of three independent experiments.