Research Article

Identification and Expression of a Putative Facilitative Urea Transporter in Three Species of True Frogs (Ranidae): Implications for Terrestrial Adaptation

Figure 1

Primary structure of newly identified urea transporters (UT). (a) Deduced amino acid sequences of UTs from Rana sylvatica (Rsy UT), R. pipiens (Rp UT), and R. septentrionalis (Rse UT) were aligned with those from R. esculenta (Re UT; accession number CAA73322), mouse UT-A2 (Mm UT-A2; accession number AAM21206), and mouse UT-B (Mm UT-B; accession number AAL47138) using ClustalW in BioEdit v7.0.9.0. Amino acids that are identical (∗), highly conserved (:), or moderately conserved (.) among all six sequences are identified. Box highlights a putative N-glycosylation site and horizontal bars indicate transmembrane regions. (b) Kyte-Doolittle hydropathy plot of Rsy-UT deduced amino acid sequences suggests the presence of ten transmembrane regions.
148276.fig.001a
(a)
148276.fig.001b
(b)