Statistical Optimization of Fermentation Process Parameters by Taguchi Orthogonal Array Design for Improved Bioethanol Production
Figure 1
SSF profile of 1% (w v−1) mixed MAA and organosolv pretreated wild grass (Achnatherum hymenoides) using unoptimized fermentation process parameters, namely, recombinant cellulase (GH5), recombinant hemicellulase (GH43) along with S. cerevisiae, C. shehatae, pH, and temperature at shake flask level showing variation of (●) cell OD measured at 600 nm, (▲) ethanol concentration (g L−1), (▼) reducing sugar (g L−1), and (○) specific activity (U mg−1) of GH5 cellulase with time (h). Similar specific activity profiles were obtained for recombinant hemicellulase (GH43) (data not shown).
