Localization of methylated H3K9, acetylated H3K9, G9a, and PCNA in the testis. Confocal images of immunohistochemical detection of H3K9me1, H3K9me2, H3K9me3, H3K9Ac, G9a, and proliferating cell nuclear antigen (PCNA) in the testis are shown. The testis was fixed in PFA for 3 h, 8 h, and 23 h fixation durations and was embedded in paraffin. Sections were treated with antigen retrieval (AR (+)) or without (AR (−)). All panels are shown as 4′,6-diamidino-2-phenylindole (DAPI)-stained images (left, blue), antigen-stained images (middle, Alexa488, green), and merged images (right). Insets of higher magnification were shown in 8 h fixation group. The same experiments are performed twice in each condition and representative photos are shown. Scale bar = 10 m.