AFM experiments. Top: force-extension curve for unfolding of Als5p. From left to right, the T domain containing the amyloid sequence unfolds first, the TR domains (sawtooth pattern), and then the invasin Ig-like region. Below: maps of Als adhesins in 1 μm² areas of the surface of live cells and heat-killed cells. The first column shows an AFM image of the tested cell, with areas mapped marked as squares. The second column shows the first mapping (area 1); 2nd column shows a 2nd mapping of the same area (area 1′); 3rd column is mapping a remote area (area 2). Reprinted with permission from Alsteens et al. [45] and Garcia et al. [41].