Research Article
Mimosine As Well As Serum Starvation Can Be Used for Cell Cycle Synchronization of Sheep Granulosa Cells
Table 3
Apoptosis of sheep granulosa cells after various treatments by TUNEL assay in primary culture (a) and 5th passage (b).
| (a) |
| | Normally growing | Confluency | Serum starvation | Cell cycle inhibitors | | 24 h | 48 h | 72 h | Mimosine | Nocodazole |
| | Viable cells | 97.3%a | 95.2% | 94.8%a | 94.3% | 93.7%b | 95.1% | 95% | | Apoptotic cells | 2.62%a | 4.7% | 5.1% | 5.6%b | 6.1%b | 4.8% | 4.9% |
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| (b) |
| | Normally growing | Confluency | Serum starvation | Cell cycle inhibitors | | 24 h | 48 h | 72 h | Mimosine | Nocodazole |
| | Viable cells | 97%
| 95.1% | 94.7% | 94.5%b | 93.3%b | 94.8% | 95% | | Apoptotic cells | 2.9%a | 4.8% | 5.2% | 5.4%b | 6.5%b | 5.1% | 4.9% |
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Within a row; percentages with different superscript alphabets are significant (). The percentages without superscript alphabets are not significant.
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