The Opportunistic Pathogen <i>Listeria monocytogenes</i>: Pathogenicity and Interaction with the Mucosal Immune System

Schuppler, Markus; Loessner, Martin J.

doi:https://doi.org/10.4061/2010/704321

International Journal of Inflammation

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Microbiota in Chronic Mucosal Inflammation

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Review Article | Open Access

Volume 2010 | Article ID 704321 | https://doi.org/10.4061/2010/704321

The Opportunistic Pathogen Listeria monocytogenes: Pathogenicity and Interaction with the Mucosal Immune System

Markus Schuppler¹and Martin J. Loessner¹

Academic Editor: Dirk Haller

Received14 Apr 2010

Accepted01 Jun 2010

Published14 Jul 2010

Abstract

Listeria monocytogenes is an opportunistic foodborne pathogen causing listeriosis, an often fatal infection leading to meningitis, sepsis, or infection of the fetus and abortion in susceptible individuals. It was recently found that the bacterium can also cause acute, self-limiting febrile gastroenteritis in healthy individuals. In the intestinal tract, L. monocytogenes penetrates the mucosa directly via enterocytes, or indirectly via invasion of Peyer’s patches. Animal models for L. monocytogenes infection have provided many insights into the mechanisms of pathogenesis, and the development of new model systems has allowed the investigation of factors that influence adaptation to the gastrointestinal environment as well as adhesion to and invasion of the intestinal mucosa. The mucosal surfaces of the gastrointestinal tract are permanently exposed to an enormous antigenic load derived from the gastrointestinal microbiota present in the human bowel. The integrity of the important epithelial barrier is maintained by the mucosal immune system and its interaction with the commensal flora via pattern recognition receptors (PRRs). Here, we discuss recent advances in our understanding of the interaction of L. monocytogenes with the host immune system that triggers the antibacterial immune responses on the mucosal surfaces of the human gastrointestinal tract.

1. Introduction

Listeria monocytogenes is a Gram-positive foodborne pathogen that is ubiquitously found in diverse environments such as soil, water, various food products, animals, and humans [1]. Infection by Listeria monocytogenes occurs almost exclusively after ingestion of contaminated food. Because the bacteria are readily inactivated at pasteurization temperature, the main source of infection represents contaminated raw food that is subjected to minimal further processing, such as soft cheeses, frankfurters, pâtés, vegetables and postprocessed contaminated milk products [2]. In individuals with impaired cell-mediated immunity such as neonates, pregnant woman, elderly persons, and immunocompromised patients suffering from transplantation events, the bacterium may cause mother-to-fetus infections, septicemia, or meningoencephalitis. Listeriosis is relatively rare and annual incidence is decreasing; in the United States from 7.7 cases per million population in 1990 to 3.1 cases per million population in 2003. In France, the incidence of listeriosis declined from 4.5 cases per million population in 1999–2000 to approximately 3.4 in 2002–2003 [3]. Although the incidence is low, the high mortality rates (about 30%) associated with listeriosis make L. monocytogenes one of the most deadly human food-borne pathogens. In contrast to the severe invasive disease recent outbreaks demonstrated that infection of healthy individuals with L. monocytogenes often leads to the development of a febrile gastroenteritis [4].

The organisms are well adapted to the conditions in the gastrointestinal tract and pursue different strategies to counteract changes in acidity, osmolarity, oxygen tension, or the challenging effects of antimicrobial peptides and bile. The finding that the bacteria are able to colonize and persist in the gallbladder [5] suggests the occurrence of long-term and chronic infections and demonstrates the ability of pathogenic Listeria to survive within the various microenvironments of the gastrointestinal tract.

Although other animals, such as guinea pigs, seem to be better suited to study the immune response to L. monocytogenes mice have been proven the most useful model for immunological studies due to availability of knock-out mice deficient in specific genes. Hence, most of our knowledge of how the immune system functions has been learned from experimental infections of mice using L. monocytogenes and the subsequent analysis of the innate and adaptive immune responses [6]. The molecules that function as pattern recognition receptors (PRRs) on epithelial cells, macrophages, and dendritic cells (DCs), thereby triggering the innate immune system after contact to bacterial pathogens, comprise Toll-like receptors (TLRs) and nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs). The recognition of pathogen-associated molecular patterns (PAMPs) by the PRRs on mucosal cells drives the activation of subsequent signaling cascades including NF-B, interferon (IFN) response factors (IRFs), activator protein 1 (AP1), and mitogen-activated protein kinases that promote the induction of proinflammatory cytokines and antimicrobial peptides, as well as the maintenance of epithelial barrier function and epithelial cell proliferation [7]. The vast amount of knowledge that has been gathered through proteomic and transcriptomic approaches makes L. monocytogenes one of the best-studied bacterial pathogens for investigations on the interplay of intracellular pathogens and the intestinal immune system. In this paper, we focus on the recent developments in the analysis of the interaction between L. monocytogenes and the mucosal immune system of the host gastrointestinal tract.

2. Adaptation of Listeria monocytogenes to the Conditions of the GI Tract

Along the gastrointestinal tract L. monocytogenes cells have to face a hostile environment characterized by altered osmolarity, low oxygen pressure, low pH, and presence of bile. In a recent article Lungu et al. [8] reconsider growth, survival, proliferation and pathogenesis of L. monocytogenes under low oxygen or anaerobic conditions. Other studies have shown that L. monocytogenes is able to launch acid resistance systems to respond to the low pH conditions in food or in the host [9, 10]. In L. monocytogenes, the glutamate decarboxylase (GAD) system confers resistance to acidic environments. Cotter and colleagues demonstrated that the expression of the GAD system correlates directly with acid tolerance of L. monocytogenes and is an absolute requirement for survival during the transmission of the stomach [9]. This system has already been associated with acid resistance in many other bacteria that need to transit the stomach before they reach their site of infection. The GAD system leads to intracellular consumption of protons by irreversible decarboxylation of extracellularly sourced glutamate and subsequent export of gamma-aminobutyrate (GABA) via a glutamate:GABA antiporter. Surprisingly, it was shown that acid-adapted L. monocytogenes were more successful in entering and proliferating in Caco-2 cells in contrast to cells that were not previously exposed to acid-stress [11].

L. monocytogenes encounters changes in the osmolarity not only in the gastrointestinal tract of its host but also in the food industry, as a preservation method or in their environmental niches. The strategy that Listeria and also other bacteria apply to respond to conditions of elevated osmolarity is the cytoplasmic accumulation of compatible solutes or osmolytes (reviewed by Sleator and Hill [13]). The finding that L. monocytogenes is able to colonize the gall bladder of infected mice demonstrates the organisms abilities to tolerate high concentrations of bile stored in this compartment [14]. The genes conferring the principal bile-resistance to L. monocytogenes are BSH and BilE [5]. This resistance has important consequences as Dussurget et al. [15] reported that bile salt hydrolase activity is essential for L. monocytogenes pathogenesis. Furthermore, carnitine uptake by the pathogen is essential for survival in the small intestine and transient colonization of the murine gastrointestinal tract [16, 17].

In the context of adaptation of L. monocytogenes to the conditions along the GI tract, the transcription factor () was identified as the key factor that triggers the manifold adaptation mechanisms. The crucial role that the protein plays in acid-tolerance was identified by Wiedmann et al. [18], whereas Becker et al. [19] independently identified as the relevant factor for adaptation of L. monocytogenes to alterations in osmolarity and temperature. Also, the genes for BSH and BilE responsible for bile-tolerance are preceded by dependent promoter sites [5, 20].

In their study, Kazmierczack et al. [21] identified fifty-five genes showing a statistically significant dependent expression after exposure of L. monocytogenes to osmotic stress. In a more recent study by Hain et al. [22], the authors report a significantly higher number of genes that were under the regulation of . They found 111 genes under negative control of and 105 genes that showed a positive dependent regulation. In L. monocytogenes contributes to both stationary- and exponential-phase acid resistance, whereas in L. innocua acid resistance is conferred by only during the exponential-phase of growth [23]. Thus, the function of seems to be strain and species dependent within the genus Listeria. Moreover, it turned out that different serotypes of L. monocytogenes reveal differences in their dependency on a functional regulon [24]. As a consequence, the resulting variations in environmental stress resistance may offer an explanation for the reported differences in the virulence traits and the survival capabilities in the host and in food for different L. monocytogenes strains [18]. The obtained results indicate that contributes to L. monocytogenes survival not only in environmental niches but also inside the host [25, 26]. This is emphasized by the finding that is required for the expression of the manifold genes that are important for the survival of L. monocytogenes within the GI tract of a host. In fact, the regulon comprises important virulence genes, encoding virulence factors such as internalin A and B [21, 22]. In murine and guinea pig models, loss of function has been shown to result in decreased virulence of L. monocytogenes after oral infection but not during systemic infection [27]. Furthermore, L. monocytogenes show an up-regulation of the sigB gene during passage of the mouse GI tract [5]. Hence, the alternative sigma factor represents a crucial prerequisite for the successful infection of a host by Listeria monocytogenes via the GI route (Figure 1).

(a)

(b)

Figure 1

Stages in the intracellular life cycle of L. monocytogenes. The cartoon (a) sketches the different stages of L. monocytogenes infection: (I) cell entry mediated by invasion factors InlA or INLB, (II) escape from phagolysosom by LLO and PlcA, (III) actin recruitment and replication, (IV) intracellular movement due to polarized actin-polymerization mediated by ActA, (V) cell-to-cell spread by formation of listeriapods, and (VI) subsequent lysis of the two-membrane vacuole by LLO and PlcB. Modified from Tilney & Portnoy [12]. The fluorescence image (b) shows the intracellular movement and cell-to-cell spread of L. monocytogenes cells (green) driven by the polarized polymerization of actin tails (red).

3. Gastroenteritis due to Listeria monocytogenes

Listeriosis is a severe foodborne disease characterized by bacteremia and meningoencephalitis in individuals with impaired cell-mediated immunity, including neonates, pregnant woman, elderly persons, and immunosuppressed patients. The incidence of listeriosis is rather low, compared to other common foodborne pathogens such as Campylobacter species, Salmonella species, Shigella species, and Vibrio species. However, the outcome is much more severe and often fatal. In fact, it represents one of the most deadly bacterial infections due to its high mean mortality rate of 20%–30%, despite early antibiotic treatment [28]. Ingestion of food contaminated with L. monocytogenes is the usual mode of transmission leading to listeriosis. Although many patients experience diarrhea antecedent to the development of bacteremia or meningoencephalitis due to L. monocytogenes infection, it was only recently that convincing evidence was obtained that L. monocytogenes can cause self-limiting, febrile gastroenteritis in healthy persons [4]. At least seven outbreaks of foodborne gastroenteritis due to L. monocytogenes infection have been reported over the last 20 years (Table 1). First evidence was obtained in 1989 when the L. monocytogenes strains isolated from blood samples of two febrile pregnant women and those from the stool samples of a person with diarrhea were shown to be identical [29]. All three patients attended the same party, and a total of 17 of the 36 attendees reported gastrointestinal complaints. More convincing evidence that L. monocytogenes could cause self-limiting gastroenteritis came from an outbreak of febrile gastroenteritis that was associated with the consumption of contaminated chocolate milk [30]. A total of 45 out of 60 persons who drank chocolate milk served in the course of a picnic developed the symptoms, and identical strains were obtained from 14 symptomatic patients, from unopened cartons of the chocolate milk, as well as from the environment of the dairy that supplied the milk [30]. One of the largest documented outbreaks of febrile gastrointestinal illness comprised 292 persons who had been hospitalized after eating in the cafeteria of two primary schools in northern Italy in 1997. The contaminated food was prepared by the same caterer and cultures from 123 stool samples and 1 blood sample from the hospitalized patients turned out to be indistinguishable to strains isolated from the food, and environmental specimens of the catering plant [31]. Therefore, L. monocytogenes should be considered to be a possible etiologic agent in outbreaks of febrile gastroenteritis when routine cultures fail to yield a pathogen.

Common symptoms observed in the effected patients included fever, watery diarrhea, nausea, headache, and pain in joints and muscles. The mechanism by which L. monocytogenes causes diarrhea is not entirely clear. However, it is likely that it is the result of direct invasion of the epithelial cells of the intestinal mucosa, as it is not known that L. monocytogenes produces enterotoxins [4]. The observed symptoms like fever as well as occasionally bloody diarrhea and bacteremia further support the hypothesis that diarrhea results from direct invasion of L. monocytogenes to the intestinal mucosal epithelium. The observation that L. monocytogenes could cause self-limiting, febrile gastroenteritis demonstrates that the pathogen induces mucosal inflammation after entering the host intestinal mucosa.

4. Adherence and Invasion of the Gastrointestinal Epithelium

There exist two principle mechanisms by which L. monocytogenes can enter into the host through the intestinal mucosa. The first route is direct invasion of the enterocytes lining the absorptive epithelium of the microvilli, leading to infection of the intestinal cells. This entry mechanism occurs only in humans and some susceptible animals (e.g., guinea pigs) that also express the correct isoform of the receptor molecules necessary for recognition by the Listeria invasion molecules, termed internalins [32]. The second entry pathway is translocation across the M-cells of Peyer’s patches [33]. This mechanism occurs also in hosts that do not express susceptible isoforms of the receptor molecules, such as mice and rats, and represents an unspecific mechanism as nonpathogenic species such as L. innocua or Bacteroides thetaiotaomicron, a prominent gut symbiont, are translocated equally. However, the latter mechanism seems to be less efficient than direct invasion of enterocytes [34].

As a first step towards invasion of the gastrointestinal epithelium the bacteria need to adhere to the surface of the epithelial cells. To enable contact with the epithelium underlying the mucus layer as the site of invasion many bacteria produce mucinases. This is not the case for L. monocytogenes which does not produce mucinases but a number of surface proteins that can bind to a specific type of human mucin [35]. Interaction with the human Muc2 isoform occurs trough the internalin proteins InlB, InlC, and InlJ. This initial interaction is thought to be an important prerequisite for the subsequent events leading to adherence and invasion of the epithelial layer [28, 36]. For various gastrointestinal pathogens, it is known that they use their flagellar structures not only as effectors of motility but also as adhesins or as a secretion apparatus. This is also not the case for L. monocytogenes which uses flagella simply for motility thereby increasing the efficacy of host invasion [37, 38].

The initial interaction of internalins with Muc2 seems not to be sufficient and further expression of proteins is necessary to warrant adherence of the pathogen to the epithelium. Listeria adhesion protein (LAP) was shown to bind to the host cell heat-shock protein 60 [39] and a specific fibronectin-binding protein (FbpA) of L. monocytogenes was identified to interact with cell surface fibronectin in the murine model [40].

Central for the pathophysiology of Listeria monocytogenes in the gastrointestinal tract is the ability to cross the intestinal barrier through invasion of enterocytes. This important event is promoted by internalin A (InlA), whereas internalin B (InlB) seems to play no direct role in invasion of cells of the gastrointestinal epithelial layer. Instead, InlB is known to mediate the invasion of hepatocytes and is required for the infection of the fetoplacental unit [46, 47]. The cellular receptor for InlA is human E-cadherin, a protein expressed at the basolateral surface of polarized enterocytes that was identified by affinity chromatography on an InlA-column [48]. The InlA E-cadherin interaction is species-specific, and was shown to rely on a single amino acid residue in the E-cadherin molecule, which is prolin in permissive species such as humans, and glutamic acid in nonpermissive species such as the mouse [34]. Although the E-cadherin of mouse and human show about 85% similarity, InlA does not interact with mouse E-cadherin. For this reason, mice are not an appropriate experimental model for oral infection with L. monocytogenes and the investigation of the pathogenic events that enable the organisms to penetrate the intestinal mucosa after ingestion of contaminated food. Consequently, the necessity for further animal models for human listeriosis led to the identification of two novel and complementary animal models. While gerbils turned out to be a natural host for L. monocytogenes, a transgenic mouse line was developed that features expression of human E-cadherin in enterocytes [49]. Use of this animal model conclusively demonstrated the role of InlA for crossing the intestinal barrier [34], and the essential and interdependent roles of InlA and InlB in feto-placental listeriosis [46]. The detailed molecular mechanisms of InlA mediated cell entry have been described and reviewed elsewhere [1, 50–53].

Upon uptake, the intracellular pathogen appears surrounded by the membranes of the phagocytic vacuole. Different phospholipases (PI-PlcA and PI-PlcB) are activated by a metalloprotease (Mpl), and cooperate with the pore-forming hemolysin listeriolysin O (LLO), which is most active at the acidic conditions (pH 5.5) of the vacuole, to confer the lysis of the phagosome membrane [54, 55]. Once the bacteria escape into the cytoplasm, they start to replicate while making use of specific transporter systems to gain carbohydrates from the host cell [56].

At the same time the pathogen is released from the phagosome, it induces the expression of ActA, a protein that triggers the nucleation and polymerization of host globular g-actin into f-actin filaments. The polarized polymerization of actin leads to a propulsive force that propels the bacteria through the cytoplasm and occasionally into the cytoplasma membrane of neighboring cells. The resulting pseudopods or “listeriapods” are then taken up (endocytosed) by the adjacent cells, thus, promoting cell-to-cell spread of the pathogen from on cell to another. The bacteria entrapped within the double membrane of the newly infected vacuole are again released by the combined action of LLO and the phospholipases, in this case the product of the plcB gene. This invasion mechanism allows L. monocytogenes to safely spread through host tissues without leaving the host cytosolic compartment, thereby protected from the host adaptive immune system. This intriguing strategy has been elucidated and thoroughly reviewed elsewhere [1, 32, 56, 57].

5. Innate Immune Responses to Listeria monocytogenes

After infection within the gastrointestinal tract, immediate immune responses are essential for the control of pathogens, such as L. monocytogenes. Activation of the innate immune system is triggered when pathogen-associated molecular patterns (PAMPs) engage pattern recognition receptors (PRRs) on intestinal epithelial cells (IECs) [58]. Despite the given name, PAMPS are actually not restricted to pathogens. They are expressed by all bacteria, invasive pathogens as well as noninvasive commensals. Typical PAMPs include bacterial carbohydrates, such as lipopolysaccharide (LPS), mannose, nucleic acids (both DNA and RNA), peptidoglycan components, lipoteichoic acids, and probably many other molecules, and are able to trigger the innate immune response. Innate immunity to L. monocytogenes is primarily mediated by two types of pattern recognition receptors, the Toll-like receptors (TLRs), and the nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs). In addition, there is some experimental evidence for the involvement of scavenger receptors and a TLR-9 independent cytosolic sensor system for bacterial DNA [59].

Toll-like receptors (TLRs) are a family of transmembrane glycoproteins, of which 10 members are known to exist in humans, where they are located on the cell surface or within endosomes. Upon recognition of the presence of microbes through sensing pathogen-associated molecular patterns, TLRs can bind any of the 4 known activating adaptors: (i) Myeloid differentiating factor-88 (MyD88), (ii) MyD88 adapter-like (Mal), (iii) TIR domain-containing adapter-inducing IFN- (TRIF), and (iv) TRIF-related adapter molecule (TRAM), whereas sterile- and Armadillo repeat-containing molecule (SARM) negatively regulates TRIF signaling [60]. MyD88 appears to be the key adaptor molecule, because it is required for signaling by all TLRs with only one exception: TLR3 uses TRIF [7]. The binding of the activating adaptors results in the subsequent recruitment of IL-1R, associated kinases (IRAKs) and downstream activation of transcription factors including NF-B and IFN regulatory factor 3 (IRF3), which in turn induces the proinflammatory cytokines and type I IFNs [60].

In the intestinal mucosa, expression and localization of PRRs on IECs and DCs differ significantly from cells of other tissues. Primary human IECs constitutively express TLR3 and TLR5, but only low levels of TLR2 and TLR4. As the location of the TLRs is crucial for their function, TLRs 1, 2, 4, 5, and 6 are expressed on the cell surface to recognize extracellular microbes, whereas TLR3, 7, 8, and 9 are present on premature endosomes [7]. As mentioned above, expression of TLRs on IECs is generally low and some receptors such as TLR5 and TLR9 are located basolaterally, thus possibly preventing an interaction with PAMPs in the intestinal lumen [7]. However, several TLRs such as TLR2, TLR4, TLR5, and TLR9 are expressed on the apical side of the IECs and important for the recognition of molecules from commensal bacteria, which is crucial to trigger innate immune responses that are required to prevent exaggerated adaptive immunity to the intestinal microbiota [61]. This is an important function indicating that low-level recognition by TLRs is necessary for protection from intestinal epithelial injury [62].

Toll-like receptor 2 (TLR2) can interact with several specific ligands, including bacterial lipoproteins, lipoteichoic acids of Gram-positive bacteria such as L. monocytogenes and yeast zymosan [7]. TLR2 can form heterodimers with TLR1 and TLR6, thereby improving the recognition of the target lipoteichoic acids [63]. TLR2 is expressed on the cell surface of intestinal epithelial cells and its activation by commensal bacteria is thought to play an important role in the maintenance of the integrity of the intestinal epithelial barrier [64]. TLR2 is also expressed within phagolysosomes, thus, L. monocytogenes cells that have escaped into the host cell cytoplasm were not detected by TLR2. The importance of TLR2 signaling for early protection against L. monocytogenes is, however, inconclusive. Whereas a first study observed that L. monocytogenes infected TLR2 deficient mice were as resistant as wild-type mice, a later study using slightly different experimental settings revealed a protective effect of TLR2 during early L. monocytogenes infection [65, 66].

Toll-like receptor 5 (TLR5) can bind to a protein motif common to the flagellin protein making up the flagella from many bacteria, such as L. monocytogenes. TLR5 activation induces NF-B and stimulates TNF production, suggesting that TLR5 may serve as a general alarm system, when the gastrointestinal barrier is compromised by a broad spectrum of motile bacteria. However, activation of TLR5 located on the apical surface of IECs by flagellin leads to an increase in the expression of antiapoptotic genes. This correlates with the observed protective effects of TLR5 signaling in epithelial homeostasis and may suggest that under physiological conditions flagellin ligation of TLR5 located on the apical surface of IECs does not exert an inflammatory response. In contrast, flagellated bacteria that interact with basolateral TLR5 signal an invasion of the epithelium by bacteria, and therefore induce a strong proinflammatory response [7].

On the other hand, flagellin-deficient L. monocytogenes revealed no significant differences in virulence in infection experiments. This observation suggests that TLR5 mediated signaling may not be essential for pathogenesis and adaptive immunity after an L. monocytogenes infection in immunized animals [67].

Toll-like receptor 9 (TLR9) recognizes the CpG motifs present in bacterial DNA. In immune cells, TLR9 is exclusively localized in the endosomes. In the intestine, TLR9 was shown to be located on both, the apical and the basolateral surface of IECs. Upon activation of TLR9, IB is degraded and NF-B is activated, again resulting in a proinflammatory response. In contrast, stimulation of apical TLR9 led to the accumulation of polyubiquitinated IB in the cytoplasm, preventing NF-B activation and inflammation [7]. Currently, there is no clear evidence that TLR9 actually contributes to the control of L. monocytogenes infection; further clarification may be obtained by animals models deficient in TLR9 [6].

In conclusion, the available experimental evidence suggests that TLR2 is the most relevant TLR for recognition of L. monocytogenes cells. However, as IECs show TLR2 commensal ligand-induced activation, TLR2 is also considered to play an important role in maintaining the integrity of the intestinal epithelial barrier [64]. This view is further supported by the observation of an increased expression of both TLR2 and TLR4, in a neonatal rat model of necrotizing enterocolitis-induced mucosal injury, suggesting that TLR2 may promote intestinal inflammation under circumstances where the epithelial barrier has been compromised [68]. In conclusion, there is no doubt that TLR signaling plays an important role for maintaining the integrity and function of the intestinal epithelium during invasion by L. monocytogenes.

NOD-like receptors (NLRs) are a group of intracellular pattern recognition receptors, which are structurally composed of an N-terminal effector domain that can comprise caspase recruitment domains (CARDs) like the NODs, or a pyrin domain as in the case of NALPs (NAcht-, Leucine-rich repeat, and Pyrin domain-containing proteins). Known members of the NLR family are NOD1, NOD2, NALP1, NALP3, neuronal apoptosis inhibitory protein-5, and the ICE protease activating factor (IPAF) [69, 70]. In humans, twenty-three NLRs have been identified so far, while in mice 34 NLRs are known [71]. The NLRs are critical for mucosal innate immunity as sensors of microbial components and cell injury in the cytoplasm [72]. They mediate proinflammatory signals through activation of caspase-1 and NF-B. Activation of caspase-1 leads to cleavage and activation of proinflammatory cytokines, such as IL-1 and IL-18, as well as to apoptotic cell death. Both NOD1 and NOD2 are important for the innate immune response against L. monocytogenes, because they represent intracellular sensors of bacterial peptidoglycan components that are thought to enter cells by endocytosis through clathrin-coated pits [73]. While NOD1 is ubiquitously expressed in adult human tissues, NOD2 is expressed only in leukocytes, DCs, and epithelial cells. Activation of NOD1 and NOD2 results in the translocation of NF-B and mitogen-activated protein kinase into the nucleus, to up-regulate the transcription of proinflammatory genes and mediate antibacterial effects by the up-regulation of another group of small antibacterial peptides, the defensins [7].

The nucleotide-binding oligomerization domain 1 (NOD1) recognizes a diaminopimelic acid-containing dipeptide or tripeptide molecule generated by lysozyme action on the peptidoglycan of many Gram-negative and Gram-positive bacteria, including L. monocytogenes [74].

The nucleotide-binding oligomerization domain 2 (NOD2) is activated by muramyl dipeptide (MDP), which is another degradation product of the peptidoglycan produced by lysozyme and other (bacterial) peptidoglycan hydrolases. In intestinal Paneth cells, NOD2-mediated signaling is important for the expression of antimicrobial peptides, the cryptidins, which are able to disrupt the membrane function of most bacteria. NOD2-deficient mice revealed an abnormal development and function of Peyer’s patches resulting in increased translocation of microbes across Peyer’s patches, and increased concentrations of cytokines such as TNF-, IFN-, IL-12, and IL-14 [75]. As a consequence, NOD2-deficient mice are highly susceptible to L. monocytogenes infection via the oral route, but normally susceptible to intravenous challange [76]. This observation demonstrates the importance of NOD2 signaling to prevent infection of the intestinal mucosa by inducing antimicrobial defensins that play an important role in in vivo defence against pathogens [77]. The intestinal P glycoprotein also seems to be important for host protection against L. monocytogenes GIT infection, most likely by inhibiting absorption of the pathogen into enterocytes [78].

Several NLRs, together with caspase-1, form proinflammatory multiprotein complexes termed the “inflammasomes”. After activation, the molecules assemble and lead to multimerization of the adaptor molecule apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain (ASC). The signaling cascade results in the processing and secretion of mature IL-1 and IL-18, which are mediators for the activation of innate and adaptive immune responses [6]. NLR family members known to form inflammasomes comprise NALP1, NALP2, NALP3, and NALP4.

NALP3 forms an inflammasome complex with ASC, cardinal, and procaspase-1 [79]. The NALP3 pathway is known to be activated by L. monocytogenes infection, although the specific ligands that activate NALP3 remain unknown. On the other hand, L. monocytogenes DNA in the host cell cytoplasm is known to act as a ligand for a hitherto unknown PRR that mediates induction of IFN- through activation of interferon regulatory factor 3 (IRF3) [80]. Interestingly, cytosolic L. monocytogenes actively increase NF-B activity by expression of the virulence factors listeriolysin O (LLO) and internalin B (InlB). This strategy leads to an increased proinflammatory response and recruitment of immune cells to the site of infection. An interesting suggestion is that the increased response actually promotes spread of intracellular pathogens, by recruiting more host cells, which can serve as potential vehicles for the pathogen [81]. Furthermore, L. monocytogenes induces expression of type I interferon (IFN-) that are known to be essential for the immune system to clear viral pathogens. However, in contrast to the protective effect to virus infections, in the case of L. monocytogenes the IFN- induction results in an increase in host susceptibility to the pathogen [82]. The observed benefit might be due either to direct enhancement of bacterial growth, or more likely, to down-modulation of a part of the immune response that plays an important role in controlling bacterial growth. The latter would be supported by the observation that induction of IFN- enables L. monocytogenes to suppress macrophage activation by IFN- [82]. It was also shown that early during L. monocytogenes infection type I interferons induce T cell apoptosis, resulting in greater IL-10 secretion by phagocytic cells which in turn leads to dampening the innate immune response [83].

Autophagy contributes to innate immune defense against various intracellular bacterial pathogens [84]. When autophagy was discovered, it was thought to serve as a pathway for recycling of intracellular organelles and cytoplasmic constituents as part of cellular homeostasis [6]. For this purpose, a double membrane vacuole is formed around the target object in the cytoplasma. This vacuole is then directed to the lysosome pathway, resulting in degradation of the vacuolar content. Similarly, intracellular bacteria can be targeted and destroyed, as it is well known for intracellular pathogens such as Salmonella, Group A Streptococcus, or Mycobacterium tuberculosis [85–87]. However, other bacteria are able to evade or even exploit autophagy during infection [88]. Pathogens adapted to persistence in the cytoplasm, such as Listeria monocytogenes and Shigella flexneri, have evolved mechanisms to avoid autophagy [89, 90].

For Salmonella and Toxoplasma, it was shown that the damaged vacuole itself triggers autophagy of the pathogens [85, 91]. However, for Listeria monocytogenes infection in Drosophila melanogaster, it was demonstrated that a peptidoglycan-recognition protein, acting as an intracellular PRR, plays an essential role for autophagy protection from the pathogen [92]. Results from previous studies indicated that L. monocytogenes deploy several mechanisms to evade from autophagy [90, 93, 94], and it was speculated whether this effect is mainly due to actin-based motility, or due to masking of the bacterial cell [93]. An earlier study suggested that active bacterial protein synthesis is required to escape from autophagy in macrophages [90], and results from a more recent study led to the assumption that bacterial phospholipases (PI-PLC and PC-PLC) may play another role [93]. How the bacterial Plc enzymes are involved is not entirely clear, but they are thought to either mediate escape from the autophagosome or prevent their formation [95]. However, in a recent study, Yoshikawa et al. [96] clearly showed that during primary infection L. monocytogenes avoids autophagy by disguising itself as a host organelle. Due to the ability of ActA to recruit host cell cytoskeleton proteins such as the Arp2/3 complex and VASP, the pathogens avoid ubiquitination and p62 accumulation. Moreover, it was demonstrated that lack of actin-based motility alone is not sufficient to escape from autophagy.

Although L. monocytogenes was previously thought to primarily reside in the cytoplasm, a recent study described the presence of variant L. monocytogenes forms that replicate in macrophages, inside large, LAMP1-positive vacuoles designated as spacious Listeria-containing phagosomes (SLAPs) [97]. The formation of SLAPs seems to be promoted by inefficient LLO activity that is not sufficient for bacterial escape from phagosomes, which triggers an autophagic response to the damaged phagosome. On the other hand, the LLO leads to disruption of the proton gradient, thereby preventing fusion with lysosomes. Within the SLAPs the bacteria are able to replicate, but the replication rate is low compared to the cytoplasm [97, 98]. The reason for the impaired LLO expression of bacteria in SLAPs is unknown. However, it is known that LLO activity is low in LAMP-1 or alkaline compartments, which are the characteristics of SLAPs [98, 99]. Moreover, function of LLO can be impaired by innate immune responses, such as reactive oxygen and nitrogen intermediates, and cathepsin D [100, 101]. Because the maturation of phagosomes is quite heterogeneous, the bacteria stuck in SLAPs may be effected by other host innate factors, compared to the bacteria that managed to escape from the phagosome [95].

6. Adaptive Immune Responses to Listeria monocytogenes

Adaptive immune responses follow the initial innate immune responses and dendritic cells (DCs) represent an important link between the two immunological pathways [102]. DCs respond to different pathogens and initiate the appropriate type of T cell response needed to control the infection. In response to L. monocytogenes infection, DCs are critical in priming the T cell response, since mice depleted of DCs are unable to generate a CD8 T cell response [103]. Due to the primarily intracellular localization of L. monocytogenes, CD4 and CD8 T cells mediate most of the adaptive immune response, and are crucial for long-term immunity after initial L. monocytogenes infection. Other cell subsets may contribute by influencing the CD4 and CD8 T cell responses. Whereas innate immune cells are important for initial control of L. monocytogenes infection, T cells are required for final clearance of the pathogen. Almost any cell type that harbors L. monocytogenes in the cytoplasm can process the proteins secreted from the pathogen, by degradation and subsequent loading on MHC class I molecules, in order to present them on the cell surface to CD8 T cells. Only professional antigen-presenting cells (APCs) can present antigens derived from lysosomal degradation via the MHC class II pathway to CD4 T cells [6]. The CD8 T cells mediate the anti-Listeria immunity by two synergistic mechanisms: first, by secretion of IFN- to activate macrophages; secondly, by lysis of infected cells via perforin and granzymes, leading to the exposure of intracellular bacteria to the activated macrophages [104]. IFN- is known to be essential for host resistance to intracellular pathogens such as L. monocytogenes, as it mediates the activation of resting macrophages that more efficiently restricts the multiplication of intracellular pathogens and promotes long-term protective cellular immunity [105].

The role of CD4 T cells in the course of the control of L. monocytogenes infection is much less well understood. L. monocytogenes induces a strong T-helper type 1 response and, similar to CD8 T cells, CD4 T cells also secrete IFN-. The strong CD8 and CD4 T cell responses results in a stable population of memory T cells specific for L. monocytogenes [6].

In the intestine, NKT cells (lymphocytes expressing both NK and T cell markers) play an important role in the control of early infection with L. monocytogenes [106]. It was shown that processing and presentation of listerial antigens is mediated by a distinct population of DCs, and strong costimulation is necessary for the development of a local antigen-specific T cell response in the intestinal mucosa. This strong costimulation seems to be required to activate appropriate antilisterial T cells and to surmount tolerance within the generally immunosuppressive intestinal milieu [107].

In general, adaptive immune responses in the intestine are characterized by high numbers of IgA producing plasma cells, regulatory T cells, and IL-17 producing T cells whose development is closely linked to factors produced by PRRs expressing IECs, DCs, and macrophages. This indicates that PRR mediated recognition of ligands produced by commensal bacteria is involved in positive and negative regulation of both, innate and adaptive immunity in the intestine [7].

7. Listeria monocytogenes L-Forms

L-forms are protoplast-like variants of bacteria that lost their ability to maintain a rigid cell wall. They have been first described at the beginning of the last century and were reported for many bacterial species. After their discovery, they have been intensively studied using numerous approaches [108]. However, due to the fastidious nature of L-form bacteria and experimental difficulties using old-fashioned techniques, these studies mainly focused on morphology and physiology of L-form cells and often led to inconclusive data. This was also the case for L-forms of L. monocytogenes [109]. It was only recently that L-form research experienced a renaissance, due to new experimental systems and the application of molecular biology and state-of-the-art imaging techniques [110–112]. In a recent study, it was demonstrated that stable L-forms of L. monocytogenes are viable bacteria that are not only able to survive, but also able to replicate and multiply using a unique, previously unknown mechanism [110]. Thus, L-forms are unlikely to be just artifacts found under laboratory conditions, but seem to represent a pre-programmed, alternative phenotype of bacterial life. Of particular interest is the observation that L. monocytogenes L-forms are able to persist within macrophages, suggesting that they retain at least a part of their pathogenetic traits (Schnell et al., unpublished data). Previous results from tissue culture studies already suggested that L-forms may be able to persist within eukaryotic cells for various time periods [113, 114]. Clinical case reports about the isolation of cell wall-deficient variants in cases of persistent and recurrent bacterial infection also suggested that L-forms may serve as cryptic agents of disease in a variety of human infectious diseases [114–118]. Subsequent reversion to parental forms may lead to a damage of the host cells. In the case of L-forms, when the bacteria have completely shed their cell walls, several proteins that represent important markers for the human immune system are also lost. Therefore, the immune system may no longer be able to discern and recognize these bacteria cells. Lack of the cell wall as an important target for antibiotic treatment represents a further threat, due to the ineffectiveness of cell-wall active drugs such as -lactams and cephalosporins on L-form cells [110, 119].

8. The Possible Role of Listeria in Inflammatory Bowel Disease

Inflammatory bowel disease is a collective term for Crohn’s disease and ulcerative colitis, both immune-mediated diseases of the gastrointestinal tract which can develop in genetically susceptible individuals [120]. A potential role of L. monocytogenes in the pathogenesis of inflammatory bowel disease (IBD) has been suggested, because interference of the pathogen with NOD2-based signaling [121], and variations of NOD2/CARD15 have been shown to represent a risk factor for Crohn's disease [122]. Especially noteworthy seems the presence of L. monocytogenes at the site of colon perforation in a patient with fulminant ulcerative colitis [123]. Another study, however, reported an equal prevalence of L. monocytogenes in patients suffering from IBD and non-IBD control patients [124], suggesting a more common occurrence of the pathogen in the gastrointestinal environment. Together with the observation of a low prevalence of L. monocytogenes in biopsies from IBD patients the available data do not yet support a role of L. monocytogenes in IBD [120]. However, there is still a lot to be done to unravel any potential indirect involvement of L. monocytogenes in the pathogenesis of IBD.

Listeria monocytogenes has been employed for decades as a model organism to study host-pathogen interactions and immune responses against intracellular pathogens [125]. The many studies provide significant insight into how L. monocytogenes interacts on host mucosal surfaces of the human gastrointestinal tract with the immune system that triggers the antibacterial immune responses. Despite the vast amount of knowledge gathered on the host-pathogen interactions and the bacterial adaptations to mammalian host, it was only recently that L. monocytogenes was found to be responsible for induction of local mucosal inflammation in immuno-competent individuals, resulting in febrile gastroenteritis [4]. Hence, the availability of new and improved animal models, such as a humanized mouse model [49], will be an important prerequisite to improve the investigation of the gastrointestinal phase of L. monocytogenes infection, in order to further enhance our understanding of the interaction and the interplay of the pathogen with the host intestinal mucosa.

References

M. Hamon, H. Bierne, and P. Cossart, “Listeria monocytogenes: a multifaceted model,” Nature Reviews Microbiology, vol. 4, no. 6, pp. 423–434, 2006.
View at: Publisher Site | Google Scholar
B. Swaminathan and P. Gerner-Smidt, “The epidemiology of human listeriosis,” Microbes and Infection, vol. 9, no. 10, pp. 1236–1243, 2007.
View at: Publisher Site | Google Scholar
D. Liu, “Epidemiology,” in Handbook of Listeria monocytogenes, D. Liu, Ed., pp. 27–59, Taylor & Francis \ CRC Press, Boca Raton, Fla, USA, 2008.
View at: Google Scholar
S. T. Ooi and B. Lorber, “Gastroenteritis due to Listeria monocytogenes,” Clinical Infectious Diseases, vol. 40, no. 9, pp. 1327–1332, 2005.
View at: Publisher Site | Google Scholar
M. Begley, C. Kerr, and C. Hill, “Exposure to bile influences biofilm formation by Listeria monocytogenes,” Gut Pathogens, vol. 1, p. 11, 2009.
View at: Google Scholar
L. A. Zenewicz and H. Shen, “Innate and adaptive immune responses to Listeria monocytogenes: a short overview,” Microbes and Infection, vol. 9, no. 10, pp. 1208–1215, 2007.
View at: Publisher Site | Google Scholar
E. C. Lavelle, C. Murphy, L. A.J. O'Neill, and E. M. Creagh, “The role of TLRs, NLRs, and RLRs in mucosal innate immunity and homeostasis,” Mucosal Immunology, vol. 3, no. 1, pp. 17–28, 2010.
View at: Publisher Site | Google Scholar
B. Lungu, S. C. Ricke, and M. G. Johnson, “Growth, survival, proliferation and pathogenesis of Listeria monocytogenes under low oxygen or anaerobic conditions: a review,” Anaerobe, vol. 15, no. 1-2, pp. 7–17, 2009.
View at: Publisher Site | Google Scholar
P. D. Cotter, K. O'Reilly, and C. Hill, “Role of the glutamate decarboxylase acid resistance system in the survival of Listeria monocytogenes LO28 in low pH foods,” Journal of Food Protection, vol. 64, no. 9, pp. 1362–1368, 2001.
View at: Google Scholar
A. Ferreira, D. Sue, C. P. O'Byrne, and K. J. Boor, “Role of Listeria monocytogenesσB in survival of lethal acidic conditions and in the acquired acid tolerance response,” Applied and Environmental Microbiology, vol. 69, no. 5, pp. 2692–2698, 2003.
View at: Publisher Site | Google Scholar
M. P. Conte, G. Petrone, A. M. Di Biase, M. G. Ammendolia, F. Superti, and L. Seganti, “Acid tolerance in Listeria monocytogenes influences invasiveness of enterocyte-like cells and macrophage-like cells,” Microbial Pathogenesis, vol. 29, no. 3, pp. 137–144, 2000.
View at: Publisher Site | Google Scholar
L. G. Tilney and D. A. Portnoy, “Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes,” Journal of Cell Biology, vol. 109, no. 4, pp. 1597–1608, 1989.
View at: Google Scholar
R. D. Sleator and C. Hill, “Bacterial osmoadaptation: the role of osmolytes in bacterial stress and virulence,” FEMS Microbiology Reviews, vol. 26, no. 1, pp. 49–71, 2002.
View at: Publisher Site | Google Scholar
J. Hardy, K. P. Francis, M. DeBoer, P. Chu, K. Gibbs, and C. H. Contag, “Extracellular replication of Listeria monocytogenes in the murine gall bladder,” Science, vol. 303, no. 5659, pp. 851–853, 2004.
View at: Publisher Site | Google Scholar
O. Dussurget, D. Cabanes, P. Dehoux, M. Lecuit, C. Buchrieser, P. Glaser, and P. Cossart, “Listeria monocytogenes bile salt hydrolase is a PrfA-regulated virulence factor involved in the intestinal and hepatic phases of listeriosis,” Molecular Microbiology, vol. 45, no. 4, pp. 1095–1106, 2002.
View at: Publisher Site | Google Scholar
R. D. Sleator, J. Wouters, C. G. M. Gahan, T. Abee, and C. Hill, “Analysis of the role of OpuC, an osmolyte transport system, in salt tolerance and virulence potential of Listeria monocytogenes,” Applied and Environmental Microbiology, vol. 67, no. 6, pp. 2692–2698, 2001.
View at: Publisher Site | Google Scholar
H. H. Wemekamp-Kamphuis, J. A. Wouters, R. D. Sleator, C. G. M. Gahan, C. Hill, and T. Abee, “Multiple deletions of the osmolyte transporters BetL, Gbu, and OpuC of Listeria monocytogenes affect virulence and growth at high osmolarity,” Applied and Environmental Microbiology, vol. 68, no. 10, pp. 4710–4716, 2002.
View at: Publisher Site | Google Scholar
M. Wiedmann, T. J. Arvik, R. J. Hurley, and K. J. Boor, “General stress transcription factor δ(B) and its role in acid tolerance and virulence of Listeria monocytogenes,” Journal of Bacteriology, vol. 180, no. 14, pp. 3650–3656, 1998.
View at: Google Scholar
L. A. Becker, M. S. Çetin, R. W. Hutkins, and A. K. Benson, “Identification of the gene encoding the alternative sigma factor σ(B) from Listera monocytogenes and its role in osmotolerance,” Journal of Bacteriology, vol. 180, no. 17, pp. 4547–4554, 1998.
View at: Google Scholar
R. D. Sleator, H. H. Wemekamp-Kamphuis, C. G. M. Gahan, T. Abee, and C. Hill, “A PrfA-regulated bile exclusion system (BilE) is a novel virulence factor in Listeria monocytogenes,” Molecular Microbiology, vol. 55, no. 4, pp. 1183–1195, 2005.
View at: Publisher Site | Google Scholar
M. J. Kazmierczak, S. C. Mithoe, K. J. Boor, and M. Wiedmann, “Listeria monocytogenesσB regulates stress response and virulence functions,” Journal of Bacteriology, vol. 185, no. 19, pp. 5722–5734, 2003.
View at: Publisher Site | Google Scholar
T. Hain, H. Hossain, and H. Hossain, “Temporal transcriptomic analysis of the Listeria monocytogenes EGD-e σb regulon,” BMC Microbiology, vol. 8, article no. 20, 2008.
View at: Publisher Site | Google Scholar
S. Raengpradub, M. Wiedmann, and K. J. Boor, “Comparative analysis of the σB-dependent stress responses in Listeria monocytogenes and Listeria innocua strains exposed to selected stress conditions,” Applied and Environmental Microbiology, vol. 74, no. 1, pp. 158–171, 2008.
View at: Publisher Site | Google Scholar
S. M. Moorhead and G. A. Dykes, “The role of the sigB gene in the general stress response of Listeria monocytogenes varies between a strain of serotype 1/2a and a strain of serotype 4c,” Current Microbiology, vol. 46, no. 6, pp. 461–466, 2003.
View at: Publisher Site | Google Scholar
C. G. M. Gahan and C. Hill, “Gastrointestinal phase of Listeria monocytogenes infection,” Journal of Applied Microbiology, vol. 98, no. 6, pp. 1345–1353, 2005.
View at: Publisher Site | Google Scholar
R. D. Sleator, G. A. Francis, D. O'Beirne, C. G. M. Gahan, and C. Hill, “Betaine and carnitine uptake systems in Listeria monocytogenes affect growth and survival in foods and during infection,” Journal of Applied Microbiology, vol. 95, no. 4, pp. 839–846, 2003.
View at: Publisher Site | Google Scholar
M. R. Garner, B. L. Njaa, M. Wiedmann, and K. J. Boor, “Sigma B contributes to Listeria monocytogenes gastrointestinal infection but not to systemic spread in the guinea pig infection model,” Infection and Immunity, vol. 74, no. 2, pp. 876–886, 2006.
View at: Publisher Site | Google Scholar
S. B. Barbuddhe and T. Chakraborty, “Listeria as an enteroinvasive gastrointestinal pathogen,” Current Topics in Microbiology and Immunology, vol. 337, pp. 173–195, 2009.
View at: Google Scholar
F. X. Riedo, R. W. Pinner, and R. W. Pinner, “A point-source foodborne listeriosis outbreak: documented incubation period and possible mild illness,” Journal of Infectious Diseases, vol. 170, no. 3, pp. 693–696, 1994.
View at: Google Scholar
C. B. Dalton, C. C. Austin, and C. C. Austin, “An outbreak of gastroenteritis and fever due to Listeria monocytogenes in milk,” New England Journal of Medicine, vol. 336, no. 2, pp. 100–105, 1997.
View at: Publisher Site | Google Scholar
P. Aureli, G. C. Fiorucci, D. Caroli, G. Marchiaro, O. Novara, L. Leone, and S. Salmaso, “An outbreak of febrile gastroenteritis associated with corn contaminated by Listeria monocytogenes,” New England Journal of Medicine, vol. 342, no. 17, pp. 1236–1241, 2000.
View at: Publisher Site | Google Scholar
J. A. Vázquez-Boland, M. Kuhn, and M. Kuhn, “Listeria pathogenesis and molecular virulence determinants,” Clinical Microbiology Reviews, vol. 14, no. 3, pp. 584–640, 2001.
View at: Publisher Site | Google Scholar
S. Corr, C. Hill, and C. G. M. Gahan, “An in vitro cell-culture model demonstrates internalin- and hemolysin-independent translocation of Listeria monocytogenes across M cells,” Microbial Pathogenesis, vol. 41, no. 6, pp. 241–250, 2006.
View at: Publisher Site | Google Scholar
M. Lecuit, “Understanding how Listeria monocytogenes targets and crosses host barriers,” Clinical Microbiology and Infection, vol. 11, no. 6, pp. 430–436, 2005.
View at: Publisher Site | Google Scholar
S. K. Lindén, H. Bierne, C. Sabet, C. W. Png, T. H. Florin, M. A. McGuckin, and P. Cossart, “Listeria monocytogenes internalins bind to the human intestinal mucin MUC2,” Archives of Microbiology, vol. 190, no. 1, pp. 101–104, 2008.
View at: Publisher Site | Google Scholar
R. D. Sleator, D. Watson, C. Hill, and C. G. M. Gahan, “The interaction between Listeria monocytogenes and the host gastrointestinal tract,” Microbiology, vol. 155, no. 8, pp. 2463–2475, 2009.
View at: Publisher Site | Google Scholar
A. Bigot, H. Pagniez, and H. Pagniez, “Role of FliF and FliI of Listeria monocytogenes in flagellar assembly and pathogenicity,” Infection and Immunity, vol. 73, no. 9, pp. 5530–5539, 2005.
View at: Publisher Site | Google Scholar
H. S. O'Neil and H. Marquis, “Listeria monocytogenes flagella are used for motility, not as adhesins, to increase host cell invasion,” Infection and Immunity, vol. 74, no. 12, pp. 6675–6681, 2006.
View at: Publisher Site | Google Scholar
J. L. Wampler, K.-P. Kim, Z. Jaradat, and A. K. Bhunia, “Heat shock protein 60 acts as a receptor for the Listeria adhesion protein in Caco-2 cells,” Infection and Immunity, vol. 72, no. 2, pp. 931–936, 2004.
View at: Publisher Site | Google Scholar
S. Dramsi, F. Bourdichon, D. Cabanes, M. Lecuit, H. Fsihi, and P. Cossart, “FbpA, a novel multifunctional Listeria monocytogenes virulence factor,” Molecular Microbiology, vol. 53, no. 2, pp. 639–649, 2004.
View at: Publisher Site | Google Scholar
G. Salamina, E. Dalle Donne, and E. Dalle Donne, “A foodborne outbreak of gastroenteritis involving Listeria monocytogenes,” Epidemiology and Infection, vol. 117, no. 3, pp. 429–436, 1996.
View at: Google Scholar
M. K. Miettinen, A. Siitonen, P. Heiskanen, H. Haajanen, K. J. Björkroth, and H. J. Korkeala, “Molecular epidemiology of an outbreak of febrile gastroenteritis caused by Listeria monocytogenes in cold-smoked rainbow trout,” Journal of Clinical Microbiology, vol. 37, no. 7, pp. 2358–2360, 1999.
View at: Google Scholar
J. Sim, D. Hood, L. Finnie, M. Wilson, C. Graham, M. Brett, and J. A. Hudson, “Series of incidents of Listeria monocytogenes non-invasive febrile gastroenteritis involving ready-to-eat meats,” Letters in Applied Microbiology, vol. 35, no. 5, pp. 409–413, 2002.
View at: Publisher Site | Google Scholar
D. M. Frye, R. Zweig, and R. Zweig, “An outbreak of febrile gastroenteritis associated with delicatessen meat contaminated with Listeria monocytogenes,” Clinical Infectious Diseases, vol. 35, no. 8, pp. 943–949, 2002.
View at: Publisher Site | Google Scholar
J.J. Carrique-Mas, I. Hökeberg, and I. Hökeberg, “Febrile gastroenteritis after eating on-farm manufactured fresh cheese—an outbreak of listeriosis?” Epidemiology and Infection, vol. 130, no. 1, pp. 79–86, 2003.
View at: Publisher Site | Google Scholar
O. Disson, S. Grayo, and S. Grayo, “Conjugated action of two species-specific invasion proteins for fetoplacental listeriosis,” Nature, vol. 455, no. 7216, pp. 1114–1118, 2008.
View at: Publisher Site | Google Scholar
N. Khelef, M. Lecuit, H. Bierne, and P. Cossart, “Species specificity of the Listeria monocytogenes InlB protein,” Cellular Microbiology, vol. 8, no. 3, pp. 457–470, 2006.
View at: Publisher Site | Google Scholar
J. Mengaud, M. Lecuit, M. Lebrun, F. Nato, J.-C. Mazie, and P. Cossart, “Antibodies to the leucine-rich repeat region of internalin block entry of Listeria monocytogenes into cells expressing E-cadherin,” Infection and Immunity, vol. 64, no. 12, pp. 5430–5433, 1996.
View at: Google Scholar
M. Lecuit, S. Vandormael-Pournin, and S. Vandormael-Pournin, “A transgenic model for listeriosis: role of internalin in crossing the intestinal barrier,” Science, vol. 292, no. 5522, pp. 1722–1725, 2001.
View at: Publisher Site | Google Scholar
H. Bierne, C. Sabet, N. Personnic, and P. Cossart, “Internalins: a complex family of leucine-rich repeat-containing proteins in Listeria monocytogenes,” Microbes and Infection, vol. 9, no. 10, pp. 1156–1166, 2007.
View at: Publisher Site | Google Scholar
M. Bonazzi, M. Lecuit, and P. Cossart, “Listeria monocytogenes internalin and E-cadherin: from structure to pathogenesis,” Cellular Microbiology, vol. 11, no. 5, pp. 693–702, 2009.
View at: Publisher Site | Google Scholar
K. Ireton, “Entry of the bacterial pathogen Listeria monocytogenes into mammalian cells,” Cellular Microbiology, vol. 9, no. 6, pp. 1365–1375, 2007.
View at: Publisher Site | Google Scholar
S. Seveau, J. Pizarro-Cerda, and P. Cossart, “Molecular mechanisms exploited by Listeria monocytogenes during host cell invasion,” Microbes and Infection, vol. 9, no. 10, pp. 1167–1175, 2007.
View at: Publisher Site | Google Scholar
H. Marquis, V. Doshi, and D. A. Portnoy, “The broad-range phospholipase C and a metalloprotease mediate listeriolysin O-independent escape of Listeria monocytogenes from a primary vacuole in human epithelial cells,” Infection and Immunity, vol. 63, no. 11, pp. 4531–4534, 1995.
View at: Google Scholar
G. A. Smith, H. Marquis, S. Jones, N. C. Johnston, D. A. Portnoy, and H. Goldfine, “The two distinct phospholipases C of Listeria monocytogenes have overlapping roles in escape from a vacuole and cell-to-cell spread,” Infection and Immunity, vol. 63, no. 11, pp. 4231–4237, 1995.
View at: Google Scholar
M. Kuhn, M. Scortti, and J.-A. Vázquez-Boland, “Pathogenesis,” in Handbook of Listeria monocytogenes, D. Liu, Ed., pp. 97–136, Taylor & Francis \ CRC Press, Boca Raton, Fla, USA, 2008.
View at: Google Scholar
O. Dussurget, J. Pizarro-Cerda, and P. Cossart, “Molecular determinants of Listeria monocytogenes virulence,” Annual Review of Microbiology, vol. 58, pp. 587–610, 2004.
View at: Publisher Site | Google Scholar
E. M. Creagh and L. A. J. O'Neill, “TLRs, NLRs and RLRs: a trinity of pathogen sensors that co-operate in innate immunity,” Trends in Immunology, vol. 27, no. 8, pp. 352–357, 2006.
View at: Publisher Site | Google Scholar
G. Geginat and S. Grauling-Halama, “Innate immunity,” in Handbook of Listeria monocytogenes, D. Liu, Ed., pp. 397–426, Taylor & Francis \ CRC Press, Boca Raton, Fla, USA, 2008.
View at: Google Scholar
L. A. J. O'Neill and A. G. Bowie, “The family of five: TIR-domain-containing adaptors in Toll-like receptor signalling,” Nature Reviews Immunology, vol. 7, no. 5, pp. 353–364, 2007.
View at: Publisher Site | Google Scholar
E. Slack, S. Hapfelmeier, and S. Hapfelmeier, “Innate and adaptive immunity cooperate flexibly to maintain host-microbiota mutualism,” Science, vol. 325, no. 5940, pp. 617–620, 2009.
View at: Publisher Site | Google Scholar
S. Rakoff-Nahoum, J. Paglino, F. Eslami-Varzaneh, S. Edberg, and R. Medzhitov, “Recognition of commensal microflora by toll-like receptors is required for intestinal homeostasis,” Cell, vol. 118, no. 2, pp. 229–241, 2004.
View at: Publisher Site | Google Scholar
L. H. Travassos, S. E. Girardin, D. J. Philpott, D. Blanot, M.-A. Nahori, C. Werts, and I. G. Boneca, “Toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition,” EMBO Reports, vol. 5, no. 10, pp. 1000–1006, 2004.
View at: Publisher Site | Google Scholar
E. Cario, G. Gerken, and D. K. Podolsky, “Toll-like receptor 2 enhances ZO-1-associated intestinal epithelial barrier integrity via protein kinase C,” Gastroenterology, vol. 127, no. 1, pp. 224–238, 2004.
View at: Publisher Site | Google Scholar
P. Cossart and J. Mengaud, “Listeria monocytogenes. A model system for the molecular study of intracellular parasittism,” Molecular Biology and Medicine, vol. 6, no. 5, pp. 463–474, 1989.
View at: Google Scholar
C. J. Czuprynski and J. F. Brown, “Effects of purified anti-Lyt-2 mAb treatment on murine listeriosis: comparative roles of Lyt-2+ and L3T4+ cells in resistance to primary and secondary infection, delayed-type hypersensitivity and adoptive transfer of resistance,” Immunology, vol. 71, no. 1, pp. 107–112, 1990.
View at: Google Scholar
S. S. Way, L. J. Thompson, J. E. Lopes, A. M. Hajjar, T. R. Kollmann, N. E. Freitag, and C. B. Wilson, “Characterization of flagellin expression and its role in Listeria monocytogenes infection and immunity,” Cellular Microbiology, vol. 6, no. 3, pp. 235–242, 2004.
View at: Publisher Site | Google Scholar
A. Le Mandat Schultz, A. Bonnard, F. Barreau, Y. Aigrain, C. Pierre-Louis, D. Berrebi, and M. Peuchmaur, “Expression of TLR-2, TLR-4, NOD2 and pNF-kappaB in a neonatal rat model of necrotizing enterocolitis,” PLoS ONE, vol. 2, no. 10, article no. e1102, 2007.
View at: Publisher Site | Google Scholar
L. M. Delbridge and M. X. O'Riordan, “Innate recognition of intracellular bacteria,” Current Opinion in Immunology, vol. 19, no. 1, pp. 10–16, 2007.
View at: Publisher Site | Google Scholar
S. Mariathasan and D. M. Monack, “Inflammasome adaptors and sensors: intracellular regulators of infection and inflammation,” Nature Reviews Immunology, vol. 7, no. 1, pp. 31–40, 2007.
View at: Publisher Site | Google Scholar
M. H. Shaw, T. Reimer, Y.-G. Kim, and G. Nuñez, “NOD-like receptors (NLRs): bona fide intracellular microbial sensors,” Current Opinion in Immunology, vol. 20, no. 4, pp. 377–382, 2008.
View at: Publisher Site | Google Scholar
Y.-G. Kim, J.-H. Park, M. H. Shaw, L. Franchi, N. Inohara, and G. Núñez, “The cytosolic sensors Nod1 and Nod2 are critical for bacterial recognition and host defense after exposure to Toll-like receptor ligands,” Immunity, vol. 28, no. 2, pp. 246–257, 2008.
View at: Publisher Site | Google Scholar
J. Lee, I. Tattoli, K. A. Wojtal, S. R. Vavricka, D. J. Philpott, and S. E. Girardin, “pH-dependent internalization of muramyl peptides from early endosomes enables Nod1 and Nod2 signaling,” Journal of Biological Chemistry, vol. 284, no. 35, pp. 23818–23829, 2009.
View at: Publisher Site | Google Scholar
S. E. Girardin, L. H. Travassos, and L. H. Travassos, “Peptidoglycan molecular requirements allowing detection by Nod1 and Nod2,” Journal of Biological Chemistry, vol. 278, no. 43, pp. 41702–41708, 2003.
View at: Publisher Site | Google Scholar
F. Barreau, U. Meinzer, and U. Meinzer, “CARD15/NOD2 is required for Peyer's patches homeostasis in mice,” PloS one, vol. 2, no. 6, article no. e523, 2007.
View at: Google Scholar
K. S. Kobayashi, M. Chamaillard, Y. Ogura, O. Henegariu, N. Inohara, G. Nuñez, and R. A. Flavell, “Nod2-dependent regulation of innate and adaptive immunity in the intestinal tract,” Science, vol. 307, no. 5710, pp. 731–734, 2005.
View at: Publisher Site | Google Scholar
C. T. Gottlieb, L. E. Thomsen, H. Ingmer, P. H. Mygind, H.-H. Kristensen, and L. Gram, “Antimicrobial peptides effectively kill a broad spectrum of Listeria monocytogenes and Staphylococcus aureus strains independently of origin, sub-type, or virulence factor expression,” BMC Microbiology, vol. 8, article no. 205, 2008.
View at: Publisher Site | Google Scholar
B. L. Neudeck, J. M. Loeb, N. G. Faith, and C. J. Czuprynski, “Intestinal P glycoprotein acts as a natural defense mechanism against Listeria monocytogenes,” Infection and Immunity, vol. 72, no. 7, pp. 3849–3854, 2004.
View at: Publisher Site | Google Scholar
F. Martinon, V. Pétrilli, A. Mayor, A. Tardivel, and J. Tschopp, “Gout-associated uric acid crystals activate the NALP3 inflammasome,” Nature, vol. 440, no. 7081, pp. 237–241, 2006.
View at: Publisher Site | Google Scholar
D. B. Stetson and R. Medzhitov, “Recognition of cytosolic DNA activates an IRF3-dependent innate immune response,” Immunity, vol. 24, no. 1, pp. 93–103, 2006.
View at: Publisher Site | Google Scholar
S. Kaya, A. Lilienbaum, O. Join-Lambert, X. Li, A. Israël, and P. Berche, “Listeriolysin O secreted by Listeria monocytogenes induces NF-κB signalling by activating the IκB kinase complex,” Molecular Microbiology, vol. 44, no. 5, pp. 1407–1419, 2002.
View at: Publisher Site | Google Scholar
M. Rayamajhi, J. Humann, K. Penheiter, K. Andreasen, and L. L. Lenz, “Induction of IFN-αβ enables Listeria monocytogenes to suppress macrophage activation by IFN-γ,” Journal of Experimental Medicine, vol. 207, no. 2, pp. 327–337, 2010.
View at: Publisher Site | Google Scholar
J. A. Carrero, B. Calderon, and E. R. Unanue, “Lymphocytes are detrimental during the early innate immune response against Listeria monocytogenes,” Journal of Experimental Medicine, vol. 203, no. 4, pp. 933–940, 2006.
View at: Publisher Site | Google Scholar
V. Deretic and B. Levine, “Autophagy, immunity, and microbial adaptations,” Cell Host and Microbe, vol. 5, no. 6, pp. 527–549, 2009.
View at: Publisher Site | Google Scholar
C. L. Birmingham, A. C. Smith, M. A. Bakowski, T. Yoshimori, and J. H. Brumell, “Autophagy controls Salmonella infection in response to damage to the Salmonella-containing vacuole,” Journal of Biological Chemistry, vol. 281, no. 16, pp. 11374–11383, 2006.
View at: Publisher Site | Google Scholar
M. G. Gutierrez, S. S. Master, S. B. Singh, G. A. Taylor, M. I. Colombo, and V. Deretic, “Autophagy is a defense mechanism inhibiting BCG and Mycobacterium tuberculosis survival in infected macrophages,” Cell, vol. 119, no. 6, pp. 753–766, 2004.
View at: Publisher Site | Google Scholar
I. Nakagawa, A. Amano, and A. Amano, “Autophagy defends cells against invading group A Streptococcus,” Science, vol. 306, no. 5698, pp. 1037–1040, 2004.
View at: Publisher Site | Google Scholar
D. Schmid and C. Münz, “Innate and adaptive immunity through autophagy,” Immunity, vol. 27, no. 1, pp. 11–21, 2007.
View at: Publisher Site | Google Scholar
M. Ogawa, T. Yoshimori, T. Suzuki, H. Sagara, N. Mizushima, and C. Sasakawa, “Escape of intracellular Shigella from autophagy,” Science, vol. 307, no. 5710, pp. 727–731, 2005.
View at: Publisher Site | Google Scholar
K. A. Rich, C. Burkett, and P. Webster, “Cytoplasmic bacteria can be targets for autophagy,” Cellular Microbiology, vol. 5, no. 7, pp. 455–468, 2003.
View at: Publisher Site | Google Scholar
S. Martens, I. Parvanova, J. Zerrahn, G. Griffiths, G. Schell, G. Reichmann, and J. C. Howard, “Disruption of Toxoplasma gondii parasitophorous vacuoles by the mouse p47-resistance GTPases,” PLoS pathogens, vol. 1, no. 3, article no. e24, 2005.
View at: Publisher Site | Google Scholar
T. Yano, S. Mita, and S. Mita, “Autophagic control of listeria through intracellular innate immune recognition in drosophila,” Nature Immunology, vol. 9, no. 8, pp. 908–916, 2008.
View at: Publisher Site | Google Scholar
C. L. Birmingham, V. Canadien, and V. Canadien, “Listeria monocytogenes evades killing by autophagy during colonization of host cells,” Autophagy, vol. 3, no. 5, pp. 442–451, 2007.
View at: Google Scholar
B. F. Py, M. M. Lipinski, and J. Yuan, “Autophagy limits Listeria monocytogenes intracellular growth in the early phase of primary infection,” Autophagy, vol. 3, no. 2, pp. 117–125, 2007.
View at: Google Scholar
C. L. Birmingham, D. E. Higgins, and J. H. Brumell, “Avoiding death by autophagy: interactions of Listeria monocytogenes with the macrophage autophagy system,” Autophagy, vol. 4, no. 3, pp. 368–371, 2008.
View at: Google Scholar
Y. Yoshikawa, M. Ogawa, and M. Ogawa, “Listeria monocytogenes ActA-mediated escape from autophagic recognition,” Nature Cell Biology, vol. 11, no. 10, pp. 1233–1240, 2009.
View at: Publisher Site | Google Scholar
C. L. Birmingham, V. Canadien, N. A. Kaniuk, B. E. Steinberg, D. E. Higgins, and J. H. Brumell, “Listeriolysin O allows Listeria monocytogenes replication in macrophage vacuoles,” Nature, vol. 451, no. 7176, pp. 350–354, 2008.
View at: Publisher Site | Google Scholar
R. Henry, L. Shaughnessy, M. J. Loessner, C. Alberti-Segui, D. E. Higgins, and J. A. Swanson, “Cytolysin-dependent delay of vacuole maturation in macrophages infected with Listeria monocytogenes,” Cellular Microbiology, vol. 8, no. 1, pp. 107–119, 2006.
View at: Publisher Site | Google Scholar
K. E. Beauregard, K.-D. Lee, R. J. Collier, and J. A. Swanson, “pH-dependent perforation of macrophage phagosomes by listeriolysin O from Listeria monocytogenes,” Journal of Experimental Medicine, vol. 186, no. 7, pp. 1159–1163, 1997.
View at: Publisher Site | Google Scholar
E. Del Cerro-Vadillo, F. Madrazo-Toca, and F. Madrazo-Toca, “Cutting edge: a novel nonoxidative phagosomal mechanism exerted by cathepsin-D controls Listeria monocytogenes intracellular growth,” Journal of Immunology, vol. 176, no. 3, pp. 1321–1325, 2006.
View at: Google Scholar
J. T. Myers, A. W. Tsang, and J. A. Swanson, “Localized reactive oxygen and nitrogen intermediates inhibit escape of Listeria monocytogenes from vacuoles in activated macrophages,” Journal of Immunology, vol. 171, no. 10, pp. 5447–5453, 2003.
View at: Google Scholar
R. Medzhitov, “Toll-like receptors and innate immunity,” Nature Reviews Immunology, vol. 1, no. 2, pp. 135–145, 2001.
View at: Google Scholar
S. Jung, D. Unutmaz, and D. Unutmaz, “In vivo depletion of CD11c⁺ dendritic cells abrogates priming of CD8⁺ T cells by exogenous cell-associated antigens,” Immunity, vol. 17, no. 2, pp. 211–220, 2002.
View at: Publisher Site | Google Scholar
J. T. Harty and V. P. Badovinac, “Influence of effector molecules on the CD8⁺ T cell response to infection,” Current Opinion in Immunology, vol. 14, no. 3, pp. 360–365, 2002.
View at: Publisher Site | Google Scholar
S. Gordon, “Alternative activation of macrophages,” Nature Reviews Immunology, vol. 3, no. 1, pp. 23–35, 2003.
View at: Publisher Site | Google Scholar
T. Ranson, S. Bregenholt, and S. Bregenholt, “Invariant Vα14+ NKT cells participate in the early response to enteric Listeria monocytogenes infection,” Journal of Immunology, vol. 175, no. 2, pp. 1137–1144, 2005.
View at: Google Scholar
A. Laouar, V. Haridas, D. Vargas, X. Zhinan, D. Chaplin, R. A. W. Van Lier, and N. Manjunath, “CD70+ antigen-presenting cells control the proliferation and differentiation of T cells in the intestinal mucosa,” Nature Immunology, vol. 6, no. 7, pp. 698–706, 2005.
View at: Publisher Site | Google Scholar
E. J. Allan, C. Hoischen, and J. Gumpert, “Bacterial L-forms,” Advances in Applied Microbiology, vol. 68, pp. 1–39, 2009.
View at: Publisher Site | Google Scholar
D. C. Edman, M. B. Pollock, and E. R. Hall, “Listeria monocytogenes L forms. I. Induction maintenance, and biological characteristics,” Journal of Bacteriology, vol. 96, no. 2, pp. 352–357, 1968.
View at: Google Scholar
S. Dell'Era, C. Buchrieser, E. Couvé, B. Schnell, Y. Briers, M. Schuppler, and M. J. Loessner, “Listeria monocytogenes l-forms respond to cell wall deficiency by modifying gene expression and the mode of division,” Molecular Microbiology, vol. 73, no. 2, pp. 306–322, 2009.
View at: Publisher Site | Google Scholar
D. Joseleau-Petit, J.-C. Liébart, J. A. Ayala, and R. D'Ari, “Unstable Escherichia coli L forms revisited: growth requires peptidoglycan synthesis,” Journal of Bacteriology, vol. 189, no. 18, pp. 6512–6520, 2007.
View at: Publisher Site | Google Scholar
M. Leaver, P. Domínguez-Cuevas, J. M. Coxhead, R. A. Daniel, and J. Errington, “Life without a wall or division machine in Bacillus subtilis,” Nature, vol. 457, no. 7231, pp. 849–853, 2009.
View at: Publisher Site | Google Scholar
B. A. Hatten, S. Y. Huang, M. L. Schulze, and S. E. Sulkin, “Electron microscopy of tissue culture cells infected with Brucella abortus,” Journal of Bacteriology, vol. 108, no. 1, pp. 535–544, 1971.
View at: Google Scholar
J. Schmitt-Slomska, E. Sacquet, and R. Caravano, “Group A streptococcal L forms. I. Persistence among inoculated mice,” Journal of Bacteriology, vol. 93, no. 1, pp. 451–455, 1967.
View at: Google Scholar
P. Charache, “Cell wall-defective bacterial variants in human disease,” Annals of the New York Academy of Sciences, vol. 174, no. 2, pp. 903–911, 1970.
View at: Google Scholar
G. J. Domingue Sr. and H. B. Woody, “Bacterial persistence and expression of disease,” Clinical Microbiology Reviews, vol. 10, no. 2, pp. 320–344, 1997.
View at: Google Scholar
A. L. Koch, “Cell wall-deficient (cwd) bacterial pathogens: could amylotrophic lateral sclerosis (als) be due to one?” Critical Reviews in Microbiology, vol. 29, no. 3, pp. 215–221, 2003.
View at: Google Scholar
N. Markova, L. Michailova, A. Vesselinova, V. Kussovski, T. Radoucheva, S. Nikolova, and I. Paskaleva, “Cell wall-deficient forms (L-forms) of Listeria monocytogenes in experimentally infected rats,” Zentralblatt fur Bakteriologie, vol. 286, no. 1, pp. 46–55, 1997.
View at: Google Scholar
A. L. Koch, “Bacterial wall as target for attack: past, present, and future research,” Clinical Microbiology Reviews, vol. 16, no. 4, pp. 673–687, 2003.
View at: Publisher Site | Google Scholar
M. Lidar, P. Langevitz, and Y. Shoenfeld, “The role of infection in inflammatory bowel disease: initiation, exacerbation and protection,” Israel Medical Association Journal, vol. 11, no. 9, pp. 558–563, 2009.
View at: Google Scholar
L. Eckmann, “Sensor molecules in intestinal innate immunity against bacterial infections,” Current Opinion in Gastroenterology, vol. 22, no. 2, pp. 95–101, 2006.
View at: Publisher Site | Google Scholar
S. R. Vavricka and G. Rogler, “New insights into the pathogenesis of Crohn's disease: are they relevant for therapeutic options?” Swiss Medical Weekly, vol. 139, no. 37-38, pp. 527–534, 2009.
View at: Google Scholar
M. Chiba, T. Fukushima, K. Koganei, N. Nakamura, and O. Masamune, “Listeria monocytogenes in the colon in a case of fulminant ulcerative colitis,” Scandinavian Journal of Gastroenterology, vol. 33, no. 7, pp. 778–782, 1998.
View at: Publisher Site | Google Scholar
P. H. Chen, G. F. Miller, and D. A. Powell, “Colitis in a female tamarin (Saguinus mystax),” Contemporary Topics in Laboratory Animal Science, vol. 39, no. 2, pp. 47–49, 2000.
View at: Google Scholar
K. M. Posfay-Barbe and E. R. Wald, “Listeriosis,” Seminars in Fetal and Neonatal Medicine, vol. 14, pp. 228–233, 2009.
View at: Publisher Site | Google Scholar

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Copyright © 2010 Markus Schuppler and Martin J. Loessner. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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