On Data Space Selection and Data Processing for Parameter Identification in a Reaction-Diffusion Model Based on FRAP Experiments

Figure 1

Experimental data from FRAP experiments [2, 3]. (a) Representative FRAP image sequence for a single cell of red algae Porphyridium cruentum for phycobilisome fluorescence. First, a fluorescence image before bleaching was detected ((A) before bleach), and then the phycobilisome fluorescence was bleached out across the middle of the cell in the vertical direction (red rectangle) and the postbleach images were recorded every 8 seconds (5 plots are displayed only). The total measurement time was 200 seconds; the length of the scale bar is 3 μm. (b) One-dimensional fluorescence profiles. The abscissa represents the position along the axis perpendicular to the bleach strip. The ordinate quantifies the corresponding values of fluorescence (in arbitrary units) averaged along the axis parallel to the bleach strip. In the central region we see the step-wise recovery of the Gaussian-shaped signals, from the lowest values of the first postbleach profile to the highest prebleach (steady-state) values on the top.

(a)

(b)