Table of Contents Author Guidelines Submit a Manuscript
Advances in Agriculture
Volume 2014 (2014), Article ID 248591, 13 pages
http://dx.doi.org/10.1155/2014/248591
Research Article

Isolation and Molecular Characterization of Potential Plant Growth Promoting Bacillus cereus GGBSTD1 and Pseudomonas spp. GGBSTD3 from Vermisources

Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram, Dindigul, Tamil Nadu 624 302, India

Received 1 May 2014; Accepted 28 July 2014; Published 1 September 2014

Academic Editor: Tibor Janda

Copyright © 2014 Balayogan Sivasankari and Marimuthu Anandharaj. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. U. S. Bhawalkar, Vermiculture Ecotechnology, Earthworm Research Institute, Pune, India, 2nd edition, 1996.
  2. S. Kiss, D. M. Bularda, and D. Radulescu, “Biological Significance of Enzymes Accumulated in Soil,” Advances in Agronomy, vol. 27, pp. 25–87, 1975. View at Publisher · View at Google Scholar · View at Scopus
  3. D. L. Karlen, M. J. Mausbach, J. W. Doran, R. G. Cline, R. F. Harris, and G. E. Schuman, “Soil quality: a concept, definition, and framework for evaluation,” Soil Science Society of America Journal, vol. 61, no. 1, pp. 4–10, 1997. View at Publisher · View at Google Scholar · View at Scopus
  4. R. M. Atiyeh, C. A. Edwards, S. Subler, and J. Metzger, “Earthworm processed organic wastes as components of horticultural potting media for growing manifold and vegetable seedlings,” Compost Science and Utilization, vol. 8, no. 3, pp. 215–223, 2000. View at Google Scholar
  5. N. Vassilev and M. Vassileva, “Biotechnological solubilization of rock phosphate on media containing agro-industrial wastes,” Applied Microbiology and Biotechnology, vol. 61, no. 5-6, pp. 435–440, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. S. A. Omar, “The role of rock-phosphate-solubilizing fungi and vesicular-arbusular-mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate,” World Journal of Microbiology and Biotechnology, vol. 14, no. 2, pp. 211–218, 1998. View at Publisher · View at Google Scholar · View at Scopus
  7. J. A. J. Paul and T. Daniel, “Standardization of sampling method for physical characterization of municipal solid waste,” Asian Journal of Water, Environment and Pollution, vol. 5, no. 1, pp. 95–98, 2007. View at Google Scholar
  8. R. Lalloo, D. Maharajh, J. Görgens, and N. Gardiner, “A downstream process for production of a viable and stable Bacillus cereus aquaculture biological agent,” Applied Microbiology and Biotechnology, vol. 86, no. 2, pp. 499–508, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. B. Sivasankari, N. Kavikumar, and M. Anandharaj, “Indole-3-acetic acid production and enhanced Plant growth promotion by Indigenous bacterial species,” Journal of Current Research in Science, vol. 1, no. 5, pp. 331–335, 2013. View at Google Scholar
  10. J. Han, L. Sun, X. Dong et al., “Characterization of a novel plant growth-promoting bacteria strain Delftia tsuruhatensis HR4 both as a diazotroph and a potential biocontrol agent against various plant pathogens,” Systematic and Applied Microbiology, vol. 28, no. 1, pp. 66–76, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. H. Rodríguez and R. Fraga, “Phosphate solubilizing bacteria and their role in plant growth promotion,” Biotechnology Advances, vol. 17, no. 4-5, pp. 319–339, 1999. View at Publisher · View at Google Scholar · View at Scopus
  12. T. Daniel and N. Karmegam, “Bio-conversion of selected leaf litters using an African epigeic earthworm, Eudrilus eugeniae,” Ecology, Environment and Conservation, vol. 5, no. 3, pp. 271–275, 1999. View at Google Scholar · View at Scopus
  13. J. Dobereiner, I. E. Marriel, and M. Nery, “Ecological distribution of Spirillum lipoferum, Beijerinck,” Canadian Journal of Microbiology, vol. 22, no. 10, pp. 1464–1473, 1976. View at Publisher · View at Google Scholar · View at Scopus
  14. R. I. Pikovskaya, “Mobilization of phosphorus in soil in connection with vital activity of some microbial species,” Microbiology, vol. 17, pp. 362–370, 1948. View at Google Scholar
  15. M. Edi Premono, A. M. Moawad, and P. L. G. Vlek, “Effect of phosphate-solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere,” Indonesian Journal of Crop Science, vol. 11, pp. 13–23, 1996. View at Google Scholar
  16. C. L. Patten and B. R. Glick, “Bacterial biosynthesis of indole-3-acetic acid,” Canadian Journal of Microbiology, vol. 42, no. 3, pp. 207–220, 1996. View at Publisher · View at Google Scholar · View at Scopus
  17. N. Amaresan, V. Jayakumar, K. Kumar, and N. Thajuddin, “Isolation and characterization of plant growth promoting endophytic bacteria and their effect on tomato (Lycopersicon esculentum) and chilli (Capsicum annuum) seedling growth,” Annals of Microbiology, vol. 62, no. 2, pp. 805–810, 2012. View at Publisher · View at Google Scholar · View at Scopus
  18. B. Schwyn and J. B. Neilands, “Universal chemical assay for the detection and determination of siderophores,” Analytical Biochemistry, vol. 160, no. 1, pp. 47–56, 1987. View at Publisher · View at Google Scholar · View at Scopus
  19. P. Rahi, P. Vyas, S. Sharma, and A. Gulati, “Plant growth promoting potential of the fungus Discosia sp. FIHB 571 from tea rhizosphere tested on chickpea, maize and pea,” Indian Journal of Microbiology, vol. 49, no. 2, pp. 128–133, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. K. Kumar, N. Amaresan, S. Bhagat, K. Madhuri, and R. C. Srivastava, “Isolation and characterization of rhizobacteria associated with coastal agricultural ecosystem of rhizosphere soils of cultivated vegetable crops,” World Journal of Microbiology and Biotechnology, vol. 27, no. 7, pp. 1625–1632, 2011. View at Publisher · View at Google Scholar · View at Scopus
  21. J. A. Smoker and S. R. Barnum, “Rapid small scale DNA isolation from filamentous cyanobacteria,” FEMS Microbiology Letters, vol. 56, no. 1, pp. 119–122, 1988. View at Publisher · View at Google Scholar
  22. U. Edwars, T. Rogall, H. Blocker, M. Emde, and E. C. Bottger, “Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA,” Nucleic Acids Research, vol. 17, no. 19, pp. 7843–7853, 1989. View at Publisher · View at Google Scholar · View at Scopus
  23. D. G. Higgins, A. J. Bleasby, and R. Fuchs, “CLUSTAL V: improved software for multiple sequence alignment,” Computer Applications in the Biosciences, vol. 8, no. 2, pp. 189–191, 1992. View at Google Scholar · View at Scopus
  24. M. Kimura, “A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences,” Journal of Molecular Evolution, vol. 16, no. 2, pp. 111–120, 1980. View at Publisher · View at Google Scholar · View at Scopus
  25. N. Saitou and M. Nei, “The neighbor-joining method: a new method for reconstructing phylogenetic trees,” Molecular Biology and Evolution, vol. 4, no. 4, pp. 406–425, 1987. View at Google Scholar · View at Scopus
  26. E. Barbu, K. Y. Lee, and R. Wahl, “Content of purine and pyrimidine base in desoxyribonucleic acid of bacteria,” Annales de l'Institut Pasteur, vol. 91, no. 2, pp. 212–224, 1956. View at Google Scholar · View at Scopus
  27. N. Sueoka, “Variation and heterogeneity of base composition of deoxyribonucleic acids: a compilation of old and new data,” Journal of Molecular Biology, vol. 3, no. 1, pp. 31–40, 1961. View at Google Scholar
  28. H. Hori and S. Osawa, “Evolutionary change in 5S rRNA secondary structure and a phylogenic tree of 352 5S rRNA species,” BioSystems, vol. 19, no. 3, pp. 163–172, 1986. View at Publisher · View at Google Scholar · View at Scopus
  29. V. Bess, “Evaluating microbiology of compost: microbial content of composting is helping the producers and growers to understand its role as a soil inoculant and plant,” BioCycle Magazine, p. 62, 1999. View at Google Scholar
  30. O. Grafff, “Preliminary experiments of vermin composting of different waste materials using Eudrilus eugeniae Kinberg,” in Workshop on the Role of Earthworms in the Stabilization of Organic Residues, M. Happelhof, Ed., pp. 178–191, Beech Leaf Press, Kalamazoo, Mich, USA, 1981. View at Google Scholar
  31. J. E. Satchell, Earthworm Ecology from Darwin to Vermiculture, Chapman and Hall, 1983.
  32. G. H. Xie, M. Y. Cai, G. C. Tao, and Y. Steinberger, “Cultivable heterotrophic N2-fixing bacterial diversity in rice fields in the Yangtze River Plain,” Biology and Fertility of Soils, vol. 37, no. 1, pp. 29–38, 2003. View at Google Scholar · View at Scopus
  33. B. B. McSpadden Gardener, “Ecology of Bacillus and Paenibacillus spp. in agricultural systems,” Phytopathology, vol. 94, no. 11, pp. 1252–1258, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. J. Dobereiner, “Biological nitrogen fixation in the tropics: social and economic contributions,” Soil Biology and Biochemistry, vol. 29, no. 5-6, pp. 771–774, 1997. View at Publisher · View at Google Scholar · View at Scopus
  35. V. M. Reis, F. L. Olivares, and J. Dobereiner, “Improved methodology for isolation of Acetobacter diazotrophicus and confirmation of its endophytic habitat,” World Journal of Microbiology and Biotechnology, vol. 10, no. 4, pp. 401–405, 1994. View at Publisher · View at Google Scholar · View at Scopus
  36. S. Khalil, N. Ayub, S. Alam, and F. Latif, “Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions,” Pakistan Journal of Biological Sciences, vol. 7, no. 2, pp. 187–196, 2004. View at Publisher · View at Google Scholar
  37. H. M. A. El-Komy, “Coimmobilization of Azospirillum lipoferum and Bacillus megaterium for successful phosphorus and nitrogen nutrition of wheat plants,” Food Technology and Biotechnology, vol. 43, no. 1, pp. 19–27, 2005. View at Google Scholar · View at Scopus
  38. N. B. Paul and W. V. B. Sundara Rao, “Phosphate-dissolving bacteria in the rhizosphere of some cultivated legumes,” Plant and Soil, vol. 35, no. 1, pp. 127–132, 1971. View at Publisher · View at Google Scholar · View at Scopus
  39. A. Beneduzi, D. Peres, L. K. Vargas, M. H. Bodanese-Zanettini, and L. M. P. Passaglia, “Evaluation of genetic diversity and plant growth promoting activities of nitrogen-fixing bacilli isolated from rice fields in South Brazil,” Applied Soil Ecology, vol. 39, no. 3, pp. 311–320, 2008. View at Publisher · View at Google Scholar · View at Scopus
  40. M. P. Raimam, U. Albino, M. F. Cruz et al., “Interaction among free-living N-fixing bacteria isolated from Drosera villosa var. villosa and AM fungi (Glomus clarum) in rice (Oryza sativa),” Applied Soil Ecology, vol. 35, no. 1, pp. 25–34, 2007. View at Publisher · View at Google Scholar · View at Scopus
  41. M. R. Swain, S. K. Naskar, and R. C. Ray, “Indole-3-acetic acid production and effect on sprouting of yam (Dioscorea rotundata L.) minisetts by Bacillus subtilis isolated from culturable cowdung microflora,” Polish Journal of Microbiology, vol. 56, no. 2, pp. 103–110, 2007. View at Google Scholar · View at Scopus
  42. T. M. Tien, M. H. Garkins, and D. H. Hubbell, “Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl Millet Pennisetum americanum (L.),” Applied and Environmental Microbiology, vol. 37, no. 5, pp. 1016–1024, 1979. View at Google Scholar
  43. A. Esitken, L. Pirlak, M. Turan, and F. Sahin, “Effects of floral and foliar application of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrition of sweet cherry,” Scientia Horticulturae, vol. 110, no. 4, pp. 324–327, 2006. View at Publisher · View at Google Scholar · View at Scopus
  44. H. Karlidag, A. Esitken, M. Turan, and F. Sahin, “Effects of root inoculation of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient element contents of leaves of apple,” Scientia Horticulturae, vol. 114, no. 1, pp. 16–20, 2007. View at Publisher · View at Google Scholar · View at Scopus
  45. C. S. Nautiyal, S. Bhadauria, P. Kumar, H. Lal, R. Mondal, and D. Verma, “Stress induced phosphate solubilization in bacteria isolated from alkaline soils,” FEMS Microbiology Letters, vol. 182, no. 2, pp. 291–296, 2000. View at Publisher · View at Google Scholar · View at Scopus
  46. A. K. Halder, A. K. Mishra, P. Bhattacharya, and P. K. Chakrabarthy, “Solubilization of inorganic phosphates by Bradyrhizobium,” Indian Journal of Experimental Biology, vol. 29, pp. 28–31, 1991. View at Google Scholar