Review Article

Chlamydial Infection and Its Role in Male Infertility

Table 1

Frequency of C. trachomatis detection among infertile males.

AuthorYearMethod of C. trachomatis testingSpecimen testedMen testing positive for Chlamydia

Nagy et al. [10]1989CultureSemen14.1% (26/184)

Bjercke and Purvis [14]1992ELISA for Chlamydia IgASemen24% (24/100)

Eggert-Kruse et al. [15] 1992ELISA for Chlamydia IgGSerum0
CultureUrethral swab0

Samra et al. [16] 1994CultureUrethral swab9.6% (13/135)
ELISA for Chlamydia IgGSerum4.4% (6/135)
ELISA for Chlamydia IgASerum1.5% (2/135)
ELISA for Chlamydia IgGSemen0
ELISA for Chlamydia IgASemen8.9% (12/135)

Munoz and Witkin [12] 1995ELISA for Chlamydia IgGSerum25% (12/48)
ELISA for Chlamydia IgASerum16.7% (8/48)
ELISA for Chlamydia IgGSemen8.3% (4/48)
ELISA for Chlamydia IgASemen29.1% (14/48)

Dieterle et al. [17] 1995PCRSemen8% (4/50)
ELISA for Chlamydia IgGSerum46% (23/50)
ELISA for Chlamydia IgASerum12% (6/50)
ELISA for Chlamydia IgGSemen12% (6/50)
ELISA for Chlamydia IgASemen16% (8/50)

Eggert-Kruse et al. [18] 1996Immunofluorescence testingSemen0
ELISA for Chlamydia IgGSemen8.1% (16/197)
ELISA for Chlamydia IgASemen18.8% (37/197)
ELISA for Chlamydia IgG and IgASerum16.2% (32/197)

Cengiz et al. [19]1997Monoclonal antibodies to ChlamydiaUrethral swab12.6% (36/284)

Eggert-Kruse et al. [20] 1997Indirect immunofluorescence assaySerum12.6% (164/1303)
Tissue cultureUrethral swab0
ELISA for Chlamydia IgGSemen1.5% (50/1303)
ELISA for Chlamydia IgASemen3.5% (46/1303)

Bornman et al. [21] 1998Tissue cultureUrethral swab18.3% (24/131)
EIAUrethral swab25.2% (33/131)
DFAUrethral swab26% (34/131)
EIAFirst void urine25.2% (33/131)
EIASemen26.7% (35/131)

Levy et al. [22] 1999ELISA for Chlamydia IgGSerum22.8% (21/92)
ELISA for Chlamydia IgASerum8.6% (8/92)
PCRSemen10.9% (10/92)

Ochsendorf et al. [23] 1999ELISA for Chlamydia IgG and IgA First void urine0.8% (1/125)
ELISA for Chlamydia IgG and IgAEjaculate0
ELISA for Chlamydia IgG and IgASeminal plasma11.2% (14/125)
ELISA for Chlamydia IgG and IgASerum31.2% (39/125)
PCRFirst void urine1.6% (2/125)
PCREjaculate1.6% (2/125)

Habermann and Krause [24]1999ELISA for Chlamydia IgG and IgA Semen90.3% (187/207)

Videau et al. [25]2001ELISA for Chlamydia IgASemen23% (23/102)

Mania-Pramanik et al. [26] 2001Direct antigen detectionUrine33.3% (5/15)
Direct antigen detectionSemen13.3% (2/15)
Direct antigen detectionSerum0
ELISA for Chlamydia IgGUrine0
Semen0
Serum46.7% (7/15)

Bollmann et al. [27] 2001ELISA for Chlamydia IgASemen38.1% (317/834)
ELISA for Chlamydia IgASerum19.1% (62/324)
ELISA for Chlamydia IgGSemen15.9% (133/834)
ELISA for Chlamydia IgGSerum67.9% (220/324)

Gdoura et al. [28] 2001Direct fluorescence antibodySemen1.1% (1/92)
Urethral swab4.3% (4/92)
Cell cultureUrethral swab1.1% (1/92)
PCRSemen16.3% (15/92)
PCRUrethral swab18.5% (17/92)
ELISA for Chlamydia IgASemen21.7% (20/92)
ELISA for Chlamydia IgAUrethral swab8.7% (8/92)

Vigil et al. [6]2002Direct immunofluorescenceSemen or urethral swab38.6% (110/284)

Eggert-Kruse et al. [29] 2003LCRUrine1.6% (11/707)
LCRSemen0.7% (5/707)

Idahl et al. [30] 2004Microimmunofluorescence for Chlamydia IgGSerum20.1% (49/243)
PCRUrine7.1% (5/70)

Hamdad-Daoudi et al. [31] 2004PCRUrine3.6% (4/111)
PCRProstatic massage0.9% (1/111)
PCRSemen2.7% (3/111)
ELISA for Chlamydia IgGSerum0.9% (1/111)
ELISA for Chlamydia IgASerum0

Hosseinzadeh et al. [32] 2004PCRSemen4.9% (31/642)
LCR Semen4.3% (28/642)

de Barbeyrac et al. [33] 2006PCRSemen0.3% (1/260)
PCRUrine0.7% (2/260)
ELISA for Chlamydia IgG and IgASerum9.5% (22/231)
ELISA for Chlamydia IgG and IgASemen4.3% (10/231)

Gdoura et al. [34] 2008PCRSemen42.3% (44/104)
PCRUrine39.4% (41/104)

Joki-Korpela et al. [35] 2009EIA to Chlamydia IgGSerum27.8% (25/90)
EIA to Chlamydia IgASerum22.2% (20/90)

Ouzounova-Raykova et al. [36] 2009CultureUrethral swab6.6% (4/60)
PCRUrethral swab8.3% (5/60)

Kokab et al. [37] 2010Strand displacement amplificationUrine3.5% (9/255)
Strand displacement amplificationSemen7.0% (18/255)
PCRUrine2.4% (6/255)
PCRSemen6.3% (16/255)

Eggert-Kruse et al. [38]2011ELISA for Chlamydia IgG Semen22.5% (39/173)
ELISA for Chlamydia IgA20.8% (36/173)
ELISA for Chlamydia IgM5.8% (10/173)

Rybar et al. [39]2011Sperm chromatin structure assaySemen13% (38/293)

Domes et al. [7]2012Strand displacement amplification assaySemen or urine0.3% (17/5588)