Review Article

Function, Structure, and Evolution of the Major Facilitator Superfamily: The LacY Manifesto

Figure 4

Site-directed alkylation of LacY mutants. The experiment demonstrates the accessibility of residues in the cytoplasmic or periplasmic cavity. (a) The ribbon structure of LacY (PDBid 2CFQ) with indicated positions of single-Cys replacements shown as black spheres. (b) Fluorescence scans of SDS-PAGE visualizing LacY mutants D68E and D68N, each in a single-Cys background at positions 60, 64, 245, or 248 labeled with tetramethylrhodamine-5-maleimide in the absence or presence of 10 mM TDG. Figure adapted from [38].
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523591.fig.004b
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