Role of Orphan Nuclear Receptor DAX-1/NR0B1 in Development, Physiology, and Disease
Figure 4
Expression of Dax-1 in mouse ES cells and effects of its knockdown on gene expression profiles. (a) Immunofluorescence showing Dax-1 expression in mouse ES cells grown in the presence of LIF (+LIF) or subjected to different differentiation protocols for 48 hours: culture in the absence of LIF (−LIF), without LIF + 1% DMSO (+DMSO), and treatment with 2 μM RA (+RA). Nuclei were counterstained with DAPI dye and images were acquired by confocal microscopy. Scale bar = 50 μm. DAPI: 4′,6-diamidino-2-phenylindole; DMSO: dimethyl sulfoxide; LIF: leukemia inhibitory factor; RA: retinoic acid. (b) Top: unsupervised clustering of genes regulated by Dax-1 knockdown in mouse ES cells using two different Dax-1 specific siRNAs (siDax-1 number 1 and siDax-1 number 3). In red, genes upregulated by Dax-1 knockdown; in green, genes downregulated by Dax-1 knockdown. Left panel: genes regulated for 24 hours after siRNA transfection; right panel: genes regulated for 48 hours after siRNA transfection. Bottom: comparison of genes regulated by Dax-1 knockdown at 24 (left) and 48 (right) hours with genes bound by Dax-1 in the study by Kim et al. [136]. From [140]. Copyright 2009 John Wiley & Sons.
