Carbofuran Modulating Functions of Acetylcholinesterase from Rat Brain In Vitro

Table 4

Effect of temperature on the rat brain AChE activity.

Temperature (°C)	AChE activity (μmole/mL/min)
4	0.057
10	0.072
20	0.072
37	0.096
40	0.087
50	0.061
60	0.008
75	0.005

Effect of varying temperatures on the activity of AChE from rat brain was observed by carrying out the enzyme assay employing 100 µg protein at pH 7.4 of phosphate buffer as described in Section 2. The enzyme was stored in phosphate buffer (50 mM, pH 7.4) containing 0.2% Triton X-100.