Table of Contents
Advances in Biology
Volume 2016 (2016), Article ID 8712470, 12 pages
Research Article

Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry

Phycology Laboratory, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal 700019, India

Received 10 August 2015; Revised 15 December 2015; Accepted 24 December 2015

Academic Editor: Antonio Randazzo

Copyright © 2016 Gour Gopal Satpati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Chlorella ellipsoidea and Chlorococcum infusionum, promising microalgae for biodiesel feedstock production, were treated with ethylenediaminetetraacetate (EDTA) and phosphorous to induce stress which was then followed by flow cytometry to study the enhanced intracellular neutral lipid content. Treatment resulted in up to a threefold increase in total lipid content of Chlorella (% at 16 days of incubation period) and more than twofold increases in Chlorococcum (% at 18 days of incubation period) under phosphorous starvation in the culture. It was observed that maximum biomass yields in Chlorella and Chlorococcum were and  g/L at 1.5 g/L of phosphorous after 20 and 18 days of incubation periods, respectively. The qualitative analyses of neutral lipid bodies under stress conditions were performed by confocal microscopy and revealed bright golden-yellow lipid droplets in stress exposed cells. Significant increase of monounsaturated fatty acids under the nutrient limited conditions was suitable to produce biodiesel. The maximum biomass (g/L) and lipid content (% dry cell weight) at different stresses showed significant results () by single-factor Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT).