Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry

Figure 5

Flow cytometric analysis of Nile red stained cells of Chlorella ellipsoidea and Chlorococcum infusionum under phosphorous starvation. (a), (d) FSC versus SSC two-dimensional dot plots. (b), (e) Flow cytograms of Nile red stained cells showing fluorescence intensity. (c), (f) Neutral lipid bodies showing yellow-gold fluorescence (the colour red indicating the cells with chlorophyll autofluorescence and violet indicating cells with neutral lipids in the scattered plots).

(a)

(b)

(c)

(d)

(e)

(f)