Figure 6: Density plot showing an example of one case where the isotype (IgG1-Alexa 488) gate settings differed between replicate plates for donor 2 (blue). In this case, the low setting for plate 9207 did not result in a significant difference between plates for the percentage of cells above the gate in the corresponding test well (CXCR5), so the gate was not modified. Plates 9206, 9207, and 9208 had 14%, 16%, and 15% percent of cells above the gate, respectively.