Fluorescence Intensity Normalisation: Correcting for Time Effects in Large-Scale Flow Cytometric Analysis
Figure 1
(a) Black crosses show nonnormalised MFIs in the CD4 memory T cell population as a function of time. The back line was fitted line to these MFI values using a loess procedure. The red line shows the normalisation coefficient estimated from the beads. (b) Repeatability plots ( pairs) for MFIs of CD25-APC cell surface expression in the CD4 memory T cell population. (c) Repeatability plots ( pairs) for CD25-APC MEF (normalised MFI) in the same cell population. For (b) and (c), each individual's blood donations were separated by at least 3 months.