Protein Bioinformatics Infrastructure for the Integration and Analysis of Multiple High-Throughput “omics” Data
Pathogen-Host Y2H protein interaction data, from Figures 5 and 6, was loaded into Cytoscape and combined with microarray and mass spectrometry data from other experiments where Bacillus anthracis was used to infect mouse macrophages. Only data that showed a significant increase or decrease in expression in the original experiments was loaded. For human interacting proteins, data from homologous mouse proteins was used. (a) The Bacillus anthracis—human protein interaction network. Triangles are Bacillus anthracis proteins, and squares are human proteins. Red indicates that an increase in expression was observed in either microarray or proteomic experiment, and green indicates a decrease in expression; (b) A list of sixteen proteins involved in eight pathogen-host interactions where a human protein showed a significant decrease in expression upon infection. Of the eight interactions three were with pathogen proteins that showed an increase in expression upon infection.
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