Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes
Table 4
Number of nontarget amplicons predicted in a multiplex reaction of tiled primers for 10 kb amplicons. As in Table 3, but for the multiplexes of the 10 kb-amplicon tiled primers.
Organism
Nontarget amplicons/total amplicons
Nontarget amplicon source organism
CCHF_S
0/160
—
CCHF_M
0/261
—
CCHF_L
0/253
—
RVF_S
0/137
—
RVF_M
0/487
—
RVF_L
0/195
—
Ebola
0/534
—
Marburg
0/123
—
Hendra
0/50
—
Nipah
0/74
—
Junin_L
0/12
—
Machupo_L
0/7
—
Junin_S
0/95
—
Machupo_S
0/32
—
JEV
0/1554
—
NW_Arena_S
1/337
Human chromosome 14 BAC C-2555K7 of library CalTech-D
