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Advances in Bioinformatics
Volume 2015, Article ID 198214, 9 pages
http://dx.doi.org/10.1155/2015/198214
Research Article

Identification of Novel Inhibitors for Tobacco Mosaic Virus Infection in Solanaceae Plants

1Data Mining & Text Mining Laboratory, Department of Bioinformatics, School of Life Sciences, Bharathiar University, Coimbatore, Tamilnadu 641046, India
2Department of Plant Molecular Biology & Bioinformatics, Centre for Plant Molecular Biology & Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu 641003, India

Received 31 July 2015; Revised 27 September 2015; Accepted 28 September 2015

Academic Editor: Huixiao Hong

Copyright © 2015 Archana Prabahar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tobacco mosaic virus (TMV) infects several crops of economic importance (e.g., tomato) and remains as one of the major concerns to the farmers. TMV enters the host cell and produces the capping enzyme RNA polymerase. The viral genome replicates further to produce multiple mRNAs which encodes several proteins, including the coat protein and an RNA-dependent RNA polymerase (RdRp), as well as the movement protein. TMV replicase domain was chosen for the virtual screening studies against small molecules derived from ligand databases such as PubChem and ChemBank. Catalytic sites of the RdRp domain were identified and subjected to docking analysis with screened ligands derived from virtual screening LigandFit. Small molecules that interact with the target molecule at the catalytic domain region amino acids, GDD, were chosen as the best inhibitors for controlling the TMV replicase activity.