FN-Identify: Novel Restriction Enzymes-Based Method for Bacterial Identification in Absence of Genome Sequencing

Table 3

Primer sequences used for 16S rRNA.

ID	Gene name	Name	Sequence	Reference
1	16S rRNA	8F	5′AGAGTTTGATCCTGGCTC AG3′	[31]
2	16S rRNA	U1492R	5′GGTTACCTTGTTACGACTT3′	[32]
3	16S rRNA	928F	5′TAAAACTYAAAKGAATTGACGGG3′	[33]
4	16S rRNA	336R	5′ACTGCTGCSYCCCGTAGGAGTCT3′	[33]
5	16S rRNA	1100F	5′YAACGAGCGCAACCC3′	[34]
6	16S rRNA	1100R	5′AGGGTTGCGCTCGTTG3′	[34]
7	16S rRNA	907R	5′CCGTCAATTCCTTTRAGTTT3′	[34]
8	16S rRNA	785F	5′GGATTAGATACCCTGGTA3′	[35]
9	16S rRNA	805R	5′GACTACCAGGGTATCTAATC3′	[36]
10	16S rRNA	515F	5′GTGCCAGCMGCCGCGGTAA3′	[34]
11	16S rRNA	518R	5′GTATTACCGCGGCTGCTGG3′	[37]
12	16S rRNA	27F	5′AGAGTTTGATCMTGGCTCAG3′	[38]
13	16S rRNA	1541R	5′AAGGAGGTGATCCAGCCGCA3′	[31]
14	HSP60	HSP60-F	5′ATGGCWAARGANNTHAARTT3′	Designed
15	HSP60	HSP60-R	5′TCDGCVACNACNGCTTCNGA3′	Designed

16S rRNA selected primers.