Research Article

Structural Prediction and Mutational Analysis of Rv3906c Gene of Mycobacterium tuberculosis H37Rv to Determine Its Essentiality in Survival

Table 2

Comparative studies by iStable (metaserver) for protein stability prediction confirm that the wild type aspartate on position 22 mutate with glycine (D22G) has the highest decrease in stability.

ā€‰Protein Stability
S. No.Mutationi -Mutant 2.0 serverMUpro serveriStable server
ā€‰ddG valuePrediction stabilityConfidence scorePrediction stabilityConfidence score

1D22G-1.28Decrease-1Decrease0.805849
2D24G-1.28Decrease-0.0645742Decrease0.786639
3D35G-0.84Decrease-0.2010039Decrease0.703789
4D120G-1.01Decrease-0.4610863Decrease0.728639
5D122G-0.87Increase0.2685563Increase0.65624
6D143G-0.89Decrease-0.2347231Decrease0.604242
7D145G-1.17Increase0.26545563Increase0.648854