Arabinogalactan Glycosyltransferases: Enzyme Assay, Protein-Protein Interaction, Subcellular Localization, and Perspectives for Application

Table 1

AtGALT29A with or without the mutagenesis of Y144. ¹Interaction with AtGALT31A as analyzed by FRET acceptor photobleaching [6, 11]; the numbers in brackets indicate the FRET efficiencies; ²frequency (%) of the subcellular targeting of the small compartments of ~0.5 μm in diameter when the construct was expressed solely or coexpressed with AtGALT31A [11]; the numbers in brackets indicate the colocalization frequency with AtGALT31A within the small compartments.

	Interaction with AtGALT31A^1	Localization to small compartments^2
		Expressed solely	Coexpressed with AtGALT31A
AtGALT29A	Yes (18%)	25%	39% (38%)
Y144A	Yes (9%)	60%	79% (66%)
Y144E	Yes (10%)	70%	66% (64%)