Abstract

Reactive Oxygen Species (ROS) are ubiquitous metabolites in all aerobic organisms. Traditionally ROS have been considered as harmful, accidental byproducts of cellular functions involving electron transport chains or electron transfer. However, it is now recognized that controlled production of ROS has significant signaling functions, for example, in pathogen defense, in the regulation of stomatal closure, or in cell-to-cell signaling. ROS formation in subcellular compartments is critical to act as “alarm” signal in the response to stress, and the concept of ROS as primarily signaling substances has emerged. The involvement of ROS in several developmental and inducible processes implies that there must be coordinated function of signaling network(s) that govern ROS responses and subsequent processes. The air pollutant ozone can be used as a useful tool to elucidate the function of apoplastic ROS: O3 degrades in cell wall into various ROS which are interpreted as ROS with signaling function inducing downstream responses. We have used ozone as a tool in mutant screens and transcript profiling-reverse genetics to identify genes involved in processes related to the signaling function of ROS. We review here our recent findings in the elucidation of apoplastic ROS sensing, signaling, and interaction with various symplastic components.

1. Introduction

Reactive Oxygen Species (ROS) are ubiquitous metabolites in all aerobic organisms. ROS include superoxide (•), hydrogen peroxide (H2O2), hydroxyl radical (•OH), singlet oxygen (1O2), and the air pollutant ozone (O3). Traditionally ROS have been considered as harmful, accidental byproducts of cellular functions involving electron transport chains or electron transfer, where electrons are accidentally passed to molecular oxygen. Consequently, cells have effective enzymatic and chemical antioxidative defenses to remove these ROS [1, 2]. The most prominent sources of ROS in plant cells are thought to be chloroplasts and mitochondria, which both contain electron transfer chains involving multiple components. Processes that take place in peroxisomes and cytoplasm also produce ROS and the apoplast can be an important site for ROS generation, although the quantity of ROS produced is several-fold less than that of the organelles.

However, it is now recognized that controlled production of ROS, for example, by the plasma membrane-localized NADPH oxidases, which produce in a controlled fashion • to the apoplastic space, has significant signaling functions [35]. ROS formation in other subcellular compartments, for example, chloroplasts, mitochondria, and peroxisomes is critical to act as “alarm” signal in the response to stress, and the concept of ROS as primarily signaling substances has emerged [2, 610]. The involvement of ROS in several developmental and inducible processes implies that there must be coordinated function of signaling network(s) that govern ROS responses and subsequent processes, although detailed descriptions of how such interactions work are lacking [11]. Furthermore, the perception of ROS produced for signaling functions is still almost completely unknown in any species.

During the last two decades different artificial systems have been developed to induce ROS production in different subcellular compartments by noninvasive methods. These include transgenic plants where the activity of the nonenzymatic or enzymatic antioxidative systems has been decreased, or where the peroxisomal catalase activity has been prevented by antisense methodology, or by expressing the H2O2-producing enzyme glycolate oxidase in the chloroplast, thus increasing the peroxisomal or chloroplastic ROS production under photorespiratory conditions [7, 1215]. These approaches have significantly increased the understanding of the mechanisms where intracellular ROS act as signaling substances. However, these approaches cannot be used to elucidate the role, significance, or the mechanisms involved in apoplastic ROS signaling and the significance of, for example, the ROS production by the plasma membrane NADPH oxidases. We have addressed this during the last two decades in noninvasive ways by using the gaseous three-atomic form of oxygen, ozone, which is itself also a ROS [6, 16, 17].

Ozone (O3) is an air pollutant produced in the atmosphere as a result of human activities. In high concentrations it causes visible damage in the affected leaves [18, 19]. The lesions visible in ozone-exposed leaves are a result of programmed cell death induced by the ROS produced from ozone degradation in the apoplastic space in the affected leaf cells [6, 16, 20, 21]. Because of this, ozone can also be used as a useful tool to probe and elucidate the function of apoplastic ROS production with the following rationale: O3 degrades into various ROS in the extracellular space of plant leaf cells; ROS formed from O3 breakdown are interpreted by the plant cells as apoplastic ROS with signaling function, which induces downstream responses [16, 22]. This makes O3 a convenient tool to study the action of apoplastic ROS in a noninvasive way (Figure 1). We have used ozone as a tool in mutant screens and transcript profiling-reverse genetics in A. thaliana and identified genes encoding proteins involved in processes related to the signaling function of ROS, such as the transcription factor-interacting nuclear protein RCD1, the chloroplast envelope membrane protein RETICULATA (RE; originally identified as RCD2), a central stomatal regulator, the long sought-after guard cell anion channel SLAC1 (originally rcd3), and an extracellular protein GRI (originally rcd5) possibly involved in ROS sensing [2326]. The same approach has also identified key components in the ascorbic acid (vitamin C) biosynthesis [27, 28].

Our mutant screens for ozone sensitivity have revealed new components in cell signaling. For example, the guard cell anion channel was not found in any direct approaches for guard cell processes—it was found in three independent mutant screens of which two were for increased ozone sensitivity and one for CO2 insensitivity [24, 29, 30]. Furthermore, we showed in a large ecotype screen good correlation between visible ozone damage and initial stomatal conductance/ozone dose that enters leaves during the very first moments of exposure [31]. Thus, ozone sensitivity is also a good marker for altered stomatal behavior and therefore can be used as a proxy for identifying components in early stomatal signaling downstream of apoplastic ROS produced normally as a result of NADPH oxidase activation. Some components upstream of the central regulatory component of stomatal movements have been identified. These include the anion channel SLAC1, the protein kinase OST1 and the protein phosphatases ABI1 and ABI2 interacting with it, and the ABA coreceptor proteins PYR/PYL/RCAR. However, several components, including the mechanisms for ROS sensing and Ca2+-related sensory and signaling components—including the plasma membrane Ca2+ influx channel—which is activated by apoplastic ROS/ozone exposure, have remained unidentified [32] and are thus a challenging target for future studies.

2. Apoplastic ROS Sensory Systems in Plants

During the last several years there has been general interest in the elucidation of the role of ROS as signaling substances and in the identification of the extracellular ROS sensing/perception systems and the immediate downstream signaling. It has been shown recently [36] that ROS act in local cell-to-cell signaling in relay manner. This has been named as “the ROS wave” that spreads relatively fast, about 8 cm/minute. The concept of “ROS wave” requires perception or sensing of ROS produced in the neighboring cells. However, ROS perception and sensing in the extracellular compartment are almost unknown in any organism. At least five different mechanisms can be proposed to be involved in apoplastic ROS sensing. Four of these are presented in Figure 1: (1) perception by an apoplastic receptor protein, which is directly modified by the ROS, or which senses ROS-induced modifications in another component present in the apoplast. We have shown indirect evidence that small apoplastic proteins [36] and plasma membrane RLKs [37, 38] can be involved in apoplastic ROS sensing. (2) Some plasma membrane ion channels, such as the K+-channel SKOR [39], are direct targets of redox regulation. It has been shown that specific Ca2+-dependent protein kinases are involved in ROS signaling. Apoplastic ROS, including O3 exposure, induce an immediate activation of a still unidentified plasma membrane Ca2+ channel [40, 41], which could as well be a target for regulation by ROS in a manner similar to SKOR. Cys residues in the channel protein itself, or other regulatory components involved in the activation of the channel extra- or intracellularly would affect Ca2+ fluxes and thus, in essence, form a sensory system for apoplastic ROS linking them directly to the activation of the downstream Ca2+-dependent protein kinases. (3) Direct transport of apoplast-formed H2O2 via aquaporins, followed by sensing in the symplasm, as has been shown for the mammalian NADPH oxidase-produced ROS. (4) There is a change in the total cellular redox balance as a result of action of the ROS on redox-active substances. The fifth possibility is the oxidation of plasma membrane lipids, which results in the formation of lipid-based signaling molecules that are further sensed. These five alternatives cannot be regarded as exclusive since they may all be involved in the various individual responses elicited by O3 in the cells affected. The identification and further elucidation of the sensory mechanisms for ROS present one of the most significant future challenges in ROS biology.

To specifically study and elucidate the mechanisms and components involved in apoplastic ROS sensing and the immediate downstream responses, a fast and specific response that can be easily observed is needed. We have shown that a rapid transient decrease in stomatal conductance (RTD) is triggered by ROS formed from the breakdown of O3 in the cell wall of guard cells. RTD is ABA independent but requires the activity of the protein kinase SnRK2.6/OST1, involving the PP2C type protein phosphatases ABI1 and ABI2, and the anion channel SLAC1 [42]. The formation of ROS from O3 in the guard cell apoplast mimics the ROS producing activity of the plasma membrane NADPH oxidases AtrbohD and AtrbohF. These NADPH oxidases are also activated in the guard cells, for example, by perception of the bacterial flagellin (or the flg22 peptide in experimental systems) by the flagellin receptor FLS2, which leads to rapid stomatal closure [43]. It can be hypothesized that the apoplastic ROS formation can act as an overriding mechanism that rapidly closes the stomatal pore, which is visible as the RTD induced by ROS formation from a short pulse of ozone. This fast response should be a useful marker in mutant screens and other approaches to identify components involved in ROS sensing and immediate downstream signaling.

Receptor-like kinases (RLKs) and transmembrane proteins that perceive signals with their extracellular domains and propagate them through the intracellular kinase domains are important components of many signaling networks. In plants RLKs control developmental and hormone responses, stomatal regulation, and stress responses, as well as defense against bacterial and fungal pathogens [4446]. Arabidopsis has more than 600 members in the RLK gene family. Although RLKs have been implicated in numerous contexts, defined functions have been assigned to only a few members in plants. ROS production and redox changes in the apoplast might also be perceived by extracellular proteins and/or plasma membrane-localized receptors (Figure 1). However, the identity and function of these receptors have so far remained elusive. One of the RLK groups, CYSTEINE-RICH RECEPTOR-LIKE KINASES (CRKs), has two conserved cysteine domains in their extracellular part (C-2x-C-8x-C; DUF26 domain). The structure of the extracellular domain of CRKs, mutant phenotypes, gene expression analysis, and thorough comprehensive transcriptional and phenotypic analysis of CRK mutants has led us and others to imply that CRKs could be involved in apoplastic ROS sensing [37, 38, 4750].

In addition to modification of extracellular domains of receptors, apoplastic secreted peptides could sense ROS (Figure 1). We have identified the extracellular GRIM REAPER (GRI) protein as a regulator of ROS-induced cell death [26]. A leucine-rich repeat (LRR) receptor-like kinase POLLEN-SPECIFIC RECEPTOR-LIKE KINASE 5 (PRK5) perceives a short peptide proteolytically cleaved from GRI [33]. In Arabidopsis leaves this small GRI-peptide is sufficient to induce cell death that requires ROS. Thus, GRI, and GRI-related apoplastic proteins could act as a novel class of sensory proteins mediating ROS-dependent information from the apoplast to nucleus and chloroplast.

3. Apoplast to Nucleus Signaling

Plants show several different responses downstream of sensing the apoplastic ROS. These range from activation of several signaling cascades and changes in gene expression to induction of programmed cell death. However, most of these responses cannot be regarded as direct or fast responses and involve complex interactions between different signaling systems. These signaling systems include second messengers such as Ca2+ connected to Ca2+-dependent protein kinases, MAP kinase cascades, hormonal signaling, and specific transcription factors that all affect the changes in gene expression detectable after apoplastic ROS sensing [47]. Arabidopsis RCD1 (Figure 2(a)), a gene/protein we originally identified in a mutant screen for ozone sensitivity [23, 51], is a member of a nuclear-localized, plant specific small SRO protein family whose members are involved in protein-protein interactions of still unknown mechanistic function [52]. Our recent results demonstrate that RCD1 seems to be involved in signaling networks that regulate quantitative changes in gene expression in response to ROS [53].

The rcd1 mutant has defective control of cell death in response to apoplastic ROS. We and others have shown that RCD1 and the other SRO proteins are also involved in several developmental and inducible processes. The protein family is highly conserved in all land plants and is involved, in addition to ROS-related processes, in other abiotic stresses [5456], in biotic stresses and pathogen defense [57], and in developmental processes such as xylem differentiation [58] and embryo development [52, 58, 59]. We recently demonstrated that all Brassicaceae have two paralogs, RCD1 and SRO1, as a result of recent partial genome duplication, while other land plants have only one orthologous protein [35]. In Arabidopsis, RCD1 and SRO1 have partially redundant function, but in the double mutant, where both paralogs are deficient, embryo development is severely affected resulting in almost unviable seeds that can be barely germinated under artificial conditions-normal germination that does not happen due to malformed embryo [52, 56, 59]. Thus, in Arabidopsis RCD1 and SRO1 can be regarded as essential proteins and presence of at least one of them is necessary for normal development. However, the molecular mechanisms by which RCD1 and SROs control plant acclimation and development have just begun to be revealed. A hypothetical model for the role of RCD1 as a positive and negative coregulator of transcription is shown in Figure 2.

The RCD protein has N-terminal nuclear localization sequences, a WWE domain found in proteins involved in ubiquitination and ADP ribosylation, a “PARP” domain poly(ADP-ribosyl)ation domain, which does not have PARP activity [34]), and a C-terminal protein-protein interaction domain (Figure 2(a)). The WWE domain is a common interaction module in proteins involved in posttranslational regulation of protein stability by ubiquitination and ADP ribosylation and has been shown to bind the iso-ADP-ribose moiety in poly(ADP-ribose) [60]. Poly(ADP-ribosyl)ation is a reversible posttranslational modification that has sometimes been compared to protein phosphorylation; the poly(ADP-ribose) polymerase (PARP) adds ADP-ribose units from NAD+ to a target and the poly(ADP-ribose) glycohydrolase (PARG) removes them (Figure 2(b)). Since the WWE domain present in several animal proteins has been demonstrated to bind PAR [60], there is also a reason to believe that the only plant WWE domain-containing proteins, RCD1 and SRO1, would do the same (Figure 2(b)).

We defined in the C-terminus of RCD1 and other SRO proteins a novel and conserved protein-protein interaction domain, RST (RCD1, SRO, and TAF4) (Figure 2(a)), through which RCD1 and SROs interact with specific transcription factors [35, 52]. However, the significance of this interaction is unknown. The most prominent RCD1-interacting protein through the RST domain is DREB2A (and also DREB2B and DREB2C, but no other member of the DREB2-protein family), a transcription factor of central importance in plant acclimation to several abiotic stresses in the ABA-independent signaling pathway [61]. The DREB2A B and C proteins have a specific domain which we showed to interact with RCD1 [34]. This domain is completely located in an exon that is a subject to developmental alternative splicing; for example, the domain is absent in DREB2A in leaves approaching senescence. Conserved amino acid residues within the RCD1-interaction motif in DREB2A 2B and 2C are required for the interaction with RCD1 [34]. Thus the interaction between RCD1 and at least some transcription factors is very specific and regulated by both partners.

The RST protein-protein interaction domain [35] is present, in addition to RCD1/SROs, also in plant TAF4-protein (TAF = TATA-binding protein-associated factors), a subunit of the general transcription factor complex TFIID. TFIID consists of the TATA-binding protein (TBP) and 12-13 TBP-associated factors (TAFs). TFIID forms the heart of the preinitiation complex and DNA-binding proteins directly contact TFIID to stimulate the rate of transcription of target genes. In the same location where plant TAF4 has the RST domain, the animal TAF4 protein contains an ETO domain (originally described in the eight twenty-one protein), which is a docking platform for positive and negative regulators of transcription. In Drosophila, for example, the ETO domain of TAF4 interacts with transcription factors and links them physically with TFIID [62]. However, it is not expected that RCD1 would be part of the TFIID complex in plants. The plant TAF4 protein interacts with TFIID with its C-terminal TAF domain, leaving the centrally located RST domain freely exposed to, for example, interaction with transcription factors. A central question is whether the RST domain in plant TAF4 and also in RCD1 and SROs is similarly a docking domain as ETO is in animals, and whether the RST domain in RCD1 is somehow involved in the control of the action of the transcription factors it interacts with (Figures 2(c) and 2(d)). The common nominator of the RCD1-interacting proteins (for which the information exists) is the regulation of protein stability by posttranslational modifications and proteolytic degradation. The RCD1 and SRO1 proteins themselves also appear to be under similar regulation. Thus, one of the questions requiring further experiments is whether RCD1 might be involved as a coregulator in affecting the stability of the interacting transcription factors and at the same time control their access to the chromatin (Figures 2(c) and 2(d)).

4. Interplay between Apoplastic and Organellar ROS Signaling Mechanisms

The two important research fields for plant productivity, photosynthesis, and stress tolerance/acclimation are generally investigated each in isolation. It is however increasingly clear that biotic and abiotic stresses are perceived in both the chloroplast and in apoplast. Moreover, particularly in biotic stress both apoplastic and chloroplastic compartments act in synergy. Indeed, the signaling networks and feedback regulation loops generated by sensing of external changes in the apoplast are likely to be transmitted via chloroplasts further to the nucleus. For example, double mutant analysis suggests a role for RCD1 in retrograde signaling from multiple organelles. We have shown that the rcd1 mutant is sensitive to apoplastic ROS but considerably more tolerant to the light-induced formation of ROS at photosystem I in the chloroplast [23]. Analysis of the photosynthetic functions in rcd1 also suggests that the mutant could be continuously acclimated to high light stress thus linking RCD1 to the regulation of both apoplastic and chloroplastic ROS. ROS formation and signaling in these two subcellular compartments are linked also in many other ways, however, mostly by indirect evidence, and thus this interaction is one of the important topics to address in ROS signaling in the future.

Our results indicate that a short apoplastic ROS burst induces ROS formation in the chloroplasts of guard cells concomitantly with the rapid and transient decrease in stomatal conductance [42]. However, the production mechanism and regulation of this chloroplastic ROS accumulation is unknown and requires further elucidation. It was shown recently that the elicitor-induced stomatal closure and H2O2 accumulation induced by pathogens were significantly reduced in the cas-1 mutantdeficient in chloroplast thylakoid-bound CAS-protein, which is crucial for proper stomatal regulation [63]. CAS was originally described as calcium sensor residing in plasma membrane [64, 65] but later shown to be a chloroplast thylakoid protein that modulates cytoplasmic Ca2+ concentration [66, 67]. Pathogen elicitors (flg22 and chitin) evoked a rapid transient increase in chloroplast stromal Ca2+ concentration and this flg22-induced stromal Ca2+ oscillation was partially dependent on CAS [63]. These data suggest that CAS could be involved in the regulation of chloroplastic ROS production after sensing of and signaling from apoplastic ROS. The electron transport chains of the chloroplasts are a logical source for ROS formation and CAS, together with its interacting components in both linear and cyclic electron transfer pathways, could regulate the processes involved. In these interactions, production of 1O2 in the light-harvesting antenna of photosystem II (LHCII) is likely to carry out critical functions, and tightly regulated photoprotective mechanisms are therefore intrinsically connected with the plants’ defense programs [63, 6770]. Similarly to the current picture in apoplastic ROS signaling, the identity of chloroplast ROS sensors, their functional characteristics, and downstream signaling components remain to be established in future studies.

Our and another mutant screen for ozone sensitivity identified a chloroplast inner envelope membrane protein RCD2/LCD1 [25, 71]. Later rcd2 and lcd1 were shown to be alleles of a classical Arabidopsis mutant reticulata (re) [72]. The RETICULATA protein is apparently a component in apoplast-chloroplast ROS signaling (Figure 1). We have shown that the extent of ozone-induced cell death in re leaves is light intensity dependent, being more severe under moderately high light and less evident under low light intensity [25]. We also found that the re mutant displays reticulated leaf pigmentation with pale green mesophyll cells and dark green bundle sheath cells that, paradoxically, under long day conditions show constitutive accumulation of ROS [25, 73]. Such pattern of ROS staining is commonly observed in suddenly light stress exposed plants [74, 75]. Although the underlying molecular mechanisms call for further analysis, these findings suggest that RE controls the outcomes of apoplast-chloroplast ROS signaling interplay.

Perhaps associated with its indisputable role in ROS signaling, RE is also required to maintain amino acid metabolism in chloroplasts and consequently, the ability to synthesize precursors for the plant hormones SA and auxin [73]. Based on this, we can position RE in regulatory network which functionally interconnects photoperiod and hormonal interactions with cross-compartmental ROS signaling which may trigger stress-induced morphogenic responses and activate protective mechanisms in plants [73, 7678].

The formation of photorespiratory ROS signals in the peroxisomes seems to play a major role in photoperiod-dependent signaling [77, 78]. Similarly to re, day length also conditions the ROS-dependent phenotype of Arabidopsis CATALASE2 (CAT2) knock-out mutants which accumulate photorespiratory H2O2 in the peroxisomes [79]. Knock-out cat2 plants show constitutive SA-dependent defense responses and cell death under long-day conditions. However, under short-day photoperiods SA signaling and cell death are not induced in the cat2 mutants, despite of the prevalence of oxidative stress [7880]. We have shown with a combination of genetic, proteomic, and metabolomic analyses that in the cat2 mutant a cytoplasmic protein phosphatase 2A regulatory subunit B′ (PP2A-B′) is required to suppress the day length-dependent, salicylic acid mediated pathogenesis responses triggered by organellar ROS signals [78, 81]. The molecular targets of PP2A phosphatases are likely to involve both metabolic enzymes and signaling components with well-known roles in cellular ROS homeostasis [78, 81]. PP2A phosphatases are predominantly trimeric and consist of a catalytic subunit C, a scaffold subunit A, and a variable regulatory subunit B, which essentially determines the target specificity of PP2A holoenzymes. Similarly to knockdown of PP2A-B′, overexpression of a calcium-dependent protein kinase AtCPK1 triggers constitutive SA signaling responses in Arabidopsis leaves [81, 82]. This raises a question whether PP2A phosphatases could act as the long sought upstream regulatory components that control CPK activity to prevent overexaggeration of stress reactions in plants [83].

5. Regulation of ROS Signaling through Reversible Protein Phosphorylation

Although reversible protein phosphorylation is a fundamental mechanism of cellular regulation, comprehensive view to the identities of kinase-phosphatase pairs with counteracting effects on stress responses in plants remains to be established. The anion channel SLAC1, as well as the protein kinase SnRK2.6/OST1, and the PP2C protein phosphatases ABI1 and ABI2 are required for the regulation of stomatal closure by ROS [42]. After the electrophysiological demonstration of the existence of guard cell anion channels 20 years ago, pharmacological experiments suggested that they are activated by phosphorylation. The identification of the SLAC1 anion channel with the ozone and CO2 sensitive mutants allowed proving this also experimentally. We and others [42, 84, 85] have shown both with heterologous expression of SLAC1 in Xenopus oocytes and in vivo that the protein kinase SnRK2.6/OST1 can phosphorylate several amino acid residues at the soluble N-terminus of SLAC1. We identified with the use of “Tilling” technique in the soluble N-terminus of SLAC1 mutations in conserved Ser/Thr residues that have a strong prediction to be targets for phosphorylation-stomatal function was impaired in these mutants [42]. Experiments with Xenopus oocytes have shown that, when coexpressed with SLAC1, in addition to SnRK2.6/OST1 [84, 85], also Ca2+-dependent protein kinases activate SLAC1-dependent ion currents in oocytes by phosphorylation of more different amino acid residues than SnRK2.6/OST1 [86, 87]. This demonstrates that several protein kinases can activate SLAC1-dependent anion currents with specific phosphorylation, which agrees well with the indirect evidence for several independent regulatory mechanisms for stomatal movements and has paved the way forward for further studies in stomatal regulation—including the elucidation of the mechanistic role of ROS therein.

6. Outlook

All living organisms react to both internal and external cues and reprogram their physiology and gene expression based on the information received; this is the central feature of growth and development as well as of environmental adaptation. These processes use the same basic scheme starting from the perception and recognition of a signal followed by the transmission of the signal leading to specific changes in physiology, gene expression, metabolite content, and growth. ROS have been established as signaling molecules in several aspects of plant life. However, the regulatory mechanisms at the biochemical level, the mechanisms downstream of ROS perception, and especially the communication and interaction between different subcellular compartments in ROS signaling are still poorly understood. Due to the importance of ROS as signaling molecules, it is central to modern plant biology to obtain a comprehensive understanding of the processes where ROS have regulatory roles. This is not only of great interest from physiological, molecular, and evolutionary research perspectives but is also of great importance for practical applications. The ultimate goal in identifying and elucidating the components of ROS signaling with ozone as a tool is to define the mechanisms and protein complexes that connect extracellular ROS-specific signals to chromatin restructuring and transcriptional regulation (extracellular space-plasma membrane-cytoplasm/chloroplast-nucleus continuum). This aims at building comprehensive plant protein interaction pathways and networks in ROS signaling and responses requires a combination of transcriptomics and proteomics approaches with analysis of protein-protein interactions and bioinformatics/systems biology, and mutant analysis.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.