Table of Contents
Advances in Botany
Volume 2015, Article ID 942761, 7 pages
http://dx.doi.org/10.1155/2015/942761
Research Article

Rhizogenesis in Cell Suspension Culture from Mango Ginger: A Source of Isosorbide and n-Hexadecanoic Acid

Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, Tamil Nadu 620020, India

Received 30 September 2014; Revised 6 February 2015; Accepted 22 February 2015

Academic Editor: Tomotsugu Koyama

Copyright © 2015 Chellappan Soundar Raju et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Curcuma amada Roxb. belongs to the monocotyledonous family Zingiberaceae. It is commonly known as mango ginger and used as a spice and valuable medicine. In this study, adventitious roots of C. amada have been successfully established from cell suspension culture. The highest percentage of adventitious root production was obtained from friable callus derived cell suspension culture. The culture conditions of adventitious root were optimized and the maximum adventitious root production was obtained in half strength MS liquid medium containing 0.3 mg L−1 IBA along with 3% of sucrose after 5 weeks of culture. Among the different initial inoculum density, the best culture condition for root growth occurred at 10 g FW of initial inoculum density. GC-MS analysis revealed that the in vitro raised adventitious roots containing two valuable bioactive compounds, isosorbide and n-hexadecanoic acid. The outcome of the present work will be helpful for the large scale cultivation of adventitious roots for the production of valuable bioactive compounds.