Mycotoxin Analysis: New Proposals for Sample Treatment

Table 2

Analytical characteristics of proposed methods.

Analytes	Matrix	Sample treatment	UHPLC chromatographic conditions	LOQs; (μg Kg^−1)	Recovery (%)	Intraday precision; (%RSD, )	Intermediate precision; (%RSD, )
AFB1, AFB2, AFG1, AFG2, OTA, FB1, FB2, T-2, HT-2, STE, CIT, DON, NIV, F-X, ZEN	Cereals and pseudocereals	QuEChERS-based extraction	Mobile phase: (A) H2O with 0.3% formic acid and 5 mM ammonium formate; (B) MeOH with 0.3% formic acid and 5 mM ammonium formate; Gradient profile: 0 min: 5% B; 1 min: 50% B; 2 min: 72% B; 4 min: 80% B and 6 min: 90% B; Column: Zorbax Eclipse Plus RRHD C18 (50 mm 2.1 mm, 1.8 m); Flow-rate: 0.4 mL min^−1; Temperature: 35°C; Injection volume: 5 L	From 0.23 (AFG1) to 233 (NIV)	From 60.0 (NIV in red rice) to 103.5 (AFG2 in white rice)	From 1.3 (FB2) to 8.8 (ZEN)	From 6.4 (HT-2) to 11.9 (FB1, FX)
OTA, FB1, FB2, T-2, HT-2, STE, CIT, DON, F-X, ZEN	Cereal syrups	QuEChERS-based extraction		From 0.45 (STE) to 75.2 (DON)	From 62.8 (STE in wheat syrup) to 100.6 (CIT in rice syrup)	From 1.3 (T-2, CIT) to 8.4 (DON)	From 2.6 (CIT) to 11.5 (OTA)
AFB1, AFB2, AFG1, AFG2, OTA, FB1, FB2, T-2, HT-2, STE, CIT, DON, F-X, ZEN	Edible nuts and seeds	QuEChERS-based extraction + DLLME		From 0.57 (OTA) to 150 (F-X)	From 61.7 (STE in almond) to 104.3 (T-2 in hazelnut)	From 0.6 (ZEN) to 8.9 (CIT)	From 6.0 (T-2) to 10.6 (DON)
AFB1, AFB2, AFG1, AFG2, OTA, FB1, FB2, T-2, HT-2, STE, CIT, DON, NIV, F-X, ZEN	Milk thistle	QuEChERS-based extraction + DLLME		From 1.50 (AFG1) to 1530 (NIV)	From 60.0 (CIT in extract) to 98.9 (AFB2 in extract)	From 4.4 (OTA) to 9.3 (AFG2)	From 5.3 (FB1) to 9.9 (T2)