Mix and Inject: Reaction Initiation by Diffusion for Time-Resolved Macromolecular Crystallography
Figure 2
(a) Schematic setup for a mix and inject time-resolved femtosecond serial crystallographic experiment at the XFEL. Nano- and microcrystals are in syringe A and substrate in syringe B. After mixing, the crystal/substrate droplet is injected into the XFEL beam. The crystal might disintegrate after the fs X-ray pulse, but diffraction occurs earlier. The diffraction pattern is damage free. (b) Time-resolved pump-probe experiment with serial femtosecond crystallography. Setup can be used to stimulate photoreceptors for photoflash experiments or to activate caged substrates. Conceptual similarities between (a) and (b) are obvious.
