Mix and Inject: Reaction Initiation by Diffusion for Time-Resolved Macromolecular Crystallography
Figure 5
Concentration profiles of free enzyme (red), (ES)1 complex (green), and (ES)2 complex (light blue); substrate in black. Vertical black dashed lines: relaxation time of the free enzyme, vertical blue dashed lines: beginning of steady state. (a) Caged substrate, activation, and diffusion times much faster than binding, instantaneous activation, (b) mixing, crystal size μm3, and diffusion times on the order of the of the turnover time, (c) mixing, crystal size μm3, and diffusion time much slower than the turnover time.
