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Analytical Cellular Pathology
Volume 15, Issue 3, Pages 145-156
http://dx.doi.org/10.1155/1997/304073

A New Method for Segmentation of Colour Images Applied to Immunohistochemically Stained Cell Nuclei

Petter Ranefall,1 Lars Egevad,2 Bo Nordin,1 and Ewert Bengtsson1

1Centre for Image Analysis, Uppsala University, Sweden
2Department of Pathology, Uppsala University, Sweden

Received 29 November 1996; Revised 1 July 1997; Accepted 7 October 1997

Copyright © 1997 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [35 citations]

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  • Jie Shu, Hao Fu, Guoping Qiu, Philip Kaye, and Mohammad Ilyas, “Segmenting overlapping cell nuclei in digital histopathology images,” 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), pp. 5445–5448, . View at Publisher · View at Google Scholar
  • Hyun Ju Choi, Ik Hwan Choi, Tae Yun Kim, and Heung Kook Choi, “A computerized program for three-dimensional visualization and quantitative analysis of cell nuclei,” Proceedings. 6th International Workshop on Enterprise Networking and Computing in Healthcare Industry - Healthcom 2004 (IEEE Cat. No.04EX842), pp. 83–87, . View at Publisher · View at Google Scholar
  • F. Arambula Cosio, J.A. Marquez Flores, M.A. Padilla Castaneda, S. Solano, and P. Tato, “Automatic counting of immunocytochemically stained cells,” Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (IEEE Cat. No.03CH37439), pp. 790–793, . View at Publisher · View at Google Scholar
  • K.Z. Mao, Peng Zhao, and Puay-Hoon Tan, “Learning-based Method for P53 Immunohistochemically Stained Cell Image Segmentation,” pp. 3264–3267, . View at Publisher · View at Google Scholar
  • Sonal Kothari, John H. Phan, Richard A. Moffitt, Todd H. Stokes, Shelby E. Hassberger, Qaiser Chaudry, Andrew N. Young, and May D. Wang, “Automatic batch-invariant color segmentation of histological cancer images,” 2011 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, pp. 657–660, . View at Publisher · View at Google Scholar
  • Jay P Ciezki, Urs O Häfeli, Priscilla Song, Nancy Urankar-Nagy, Norman B Ratliff, Lisa Rybicki, Kevin Brill, and Dominik Meier, “Parenchymal cell proliferation in coronary arteries after percutaneous transluminal coronary angioplasty: a human tissue bank study,” International Journal of Radiation Oncology*Biology*Physics, vol. 45, no. 4, pp. 963–968, 1999. View at Publisher · View at Google Scholar
  • Yi-Hua Xu, Gerald L Sattler, Harlene Edwards, and Henry C Pitot, “Nuclear-labeling index analysis (NLIA), a software package used to perform accurate automation of cell nuclear-labeling index analysis on immunohistochemically stained rat liver samples,” Computer Methods and Programs in Biomedicine, vol. 63, no. 1, pp. 55–70, 2000. View at Publisher · View at Google Scholar
  • Jonas Isaksson, Mohammad Farooque, and Yngve Olsson, “Spinal Cord Injury in ICAM-1–Deficient Mice: Assessment of Functional and Histopathological Outcome,” Journal of Neurotrauma, vol. 17, no. 4, pp. 333–344, 2000. View at Publisher · View at Google Scholar
  • Kenneth Wester, Eva Wahlund, Christer Sundstrom, Petter Ranefall, Ewert Bengtsson, Pamela Russell, Kim Ow, Per-Uno Malmstrom, and Christer Busch, “,” Applied Immunohistochemistry, vol. 8, no. 1, pp. 61–70, 2000. View at Publisher · View at Google Scholar
  • Mohammad Farooque, Jonas Isaksson, and Yngve Olsson, “Improved Recovery After Spinal Cord Injury in Neuronal Nitric Oxide Synthase-Deficient Mice But not in TNF-α-Deficient Mice,” Journal of Neurotrauma, vol. 18, no. 1, pp. 105–114, 2001. View at Publisher · View at Google Scholar
  • C Busch, Ta Hanssen, C Wagener, and B Obrink, “Down-regulation of CEACAM1 in human prostate cancer: Correlation with loss of cell polarity, increased proliferation rate, and Gleason grade 3 to 4 tr,” Human Pathology, vol. 33, no. 3, pp. 290–298, 2002. View at Publisher · View at Google Scholar
  • Guida Portela-Gomes, Lars Grimelius, Henry Johansson, Suad Efendic, Kenneth Wester, and Samy Abdel-Halim, “,” Applied Immunohistochemistry, vol. 10, no. 4, pp. 387–392, 2002. View at Publisher · View at Google Scholar
  • Guida Maria Portela-Gomes, Lars Grimelius, Henry Johansson, Suad Efendic, Kenneth Wester, and Samy M. Abdel-Halim, “Increased Expression of Adenylyl Cyclase Isoforms in the Adrenal Gland of Diabetic Goto-Kakizaki Rat,” Applied Immunohistochemistry & Molecular Morphology, vol. 10, no. 4, pp. 387–392, 2002. View at Publisher · View at Google Scholar
  • Pu Malmstrom, Zp Ren, A Sherif, M de la Torre, K Wester, and M Thorn, “Early metastatic progression of bladder carcinoma: Molecular profile of primary tumor and sentinel lymph node,” Journal Of Urology, vol. 168, no. 5, pp. 2240–2244, 2002. View at Publisher · View at Google Scholar
  • Per-Uno Malmstr??M, Zhi-Ping Ren, Amir Sherif, Manuel de la TORRE, Kenneth Wester, and Magnus Th??Rn, “Early Metastatic Progression of Bladder Carcinoma: Molecular Profile of Primary Tumor and Sentinel Lymph Node,” The Journal of Urology, pp. 2240–2244, 2002. View at Publisher · View at Google Scholar
  • Johanna ??sterberg, and Ulf Haglund, “Effect of Linomide on Gut Immune Cell Distribution and on TNF-?? in Plasma and Ascites: An Experimental Study in the Septic Rat,” Shock, vol. 18, no. 5, pp. 471–475, 2002. View at Publisher · View at Google Scholar
  • Per-Uno MalmstrÖM, Zhi-Ping Ren, Amir Sherif, Manuel de la TORRE, Kenneth Wester, and Magnus ThÖRn, “Early Metastatic Progression of Bladder Carcinoma: Molecular Profile of Primary Tumor and Sentinel Lymph Node,” The Journal of Urology, vol. 168, no. 5, pp. 2240–2244, 2002. View at Publisher · View at Google Scholar
  • Albert K.W Law, K.Y Lam, F.K Lam, Teresa K.W Wong, Jacky L.S Poon, and Francis H.Y Chan, “Image analysis system for assessment of immunohistochemically stained proliferative marker (MIB-1) in oesophageal squamous cell carcinoma,” Computer Methods and Programs in Biomedicine, vol. 70, no. 1, pp. 37–45, 2003. View at Publisher · View at Google Scholar
  • Joakim Lindblad, Carolina W�hlby, Ewert Bengtsson, and Alla Zaltsman, “Image analysis for automatic segmentation of cytoplasms and classification of Rac1 activation,” Cytometry, vol. 57A, no. 1, pp. 22–33, 2003. View at Publisher · View at Google Scholar
  • Dimitris Glotsos, Jussi Tohka, Panagiota Ravazoula, Dionisis Cavouras, and George Nikiforidis, “Automated Diagnosis Of Brain Tumours Astrocytomas Using Probabilistic Neural Network Clustering And Support Vector Machines,” International Journal of Neural Systems, vol. 15, no. 01n02, pp. 1–11, 2005. View at Publisher · View at Google Scholar
  • F. Arámbula Cosío, J. A. Márquez Flores, M. A. Padilla Castañeda, S. Solano, and P. Tato, “Automatic analysis of immunocytochemically stained tissue samples,” Medical & Biological Engineering & Computing, vol. 43, no. 5, pp. 672–677, 2005. View at Publisher · View at Google Scholar
  • Jouni Lauronen, Pekka Hayry, and Timo Paavonen, “An image analysis-based method for quantification of chronic allograft damage index parameters,” Apmis, vol. 114, no. 6, pp. 440–448, 2006. View at Publisher · View at Google Scholar
  • Kz Mao, P Zhao, and Ph Tan, “Supervised learning-based cell image segmentation for P53 immunohistochemistry,” Ieee Transactions On Biomedical Engineering, vol. 53, no. 6, pp. 1153–1163, 2006. View at Publisher · View at Google Scholar
  • Ann-Catrin Andersson, Sara Stromberg, Helena Backvall, Caroline Kampf, Mathias Uhlen, Kenneth Wester, and Fredrik Ponten, “Analysis of protein expression in cell microarrays: A tool for antibody-based proteomics,” Journal Of Histochemistry & Cytochemistry, vol. 54, no. 12, pp. 1413–1423, 2006. View at Publisher · View at Google Scholar
  • Peter J. W. Iles, G. Wayne Brodland, David A. Clausi, and Shannon M. Puddister, “Estimation of cellular fabric in embryonic epithelia,” Computer Methods in Biomechanics and Biomedical Engineering, vol. 10, no. 1, pp. 75–84, 2007. View at Publisher · View at Google Scholar
  • Hernani D. Cualing, Eric Zhong, and Lynn Moscinski, “"Virtual flow cytometry" of immunostained lymphocytes on microscopic tissue slides: iHCFlow (TM) tissue cytometry,” Cytometry Part B-Clinical Cytometry, vol. 72B, no. 1, pp. 63–76, 2007. View at Publisher · View at Google Scholar
  • H.-S. Wu, M.I. Fiel, T.D. Schiano, and J. Gil, “Image segmentation of liver fibrosis,” Journal of Microscopy, vol. 231, no. 1, pp. 70–80, 2008. View at Publisher · View at Google Scholar
  • Carlos López, Marylène Lejeune, María Teresa Salvadó, Patricia Escrivà, Ramón Bosch, Lluis E. Pons, Tomás Álvaro, Jordi Roig, Xavier Cugat, Jordi Baucells, and Joaquín Jaén, “Automated quantification of nuclear immunohistochemical markers with different complexity,” Histochemistry and Cell Biology, vol. 129, no. 3, pp. 379–387, 2008. View at Publisher · View at Google Scholar
  • Hans Polzer, Florian Haasters, Wolf Christian Prall, Maximilian Michael Saller, Elias Volkmer, Inga Drosse, Wolf Mutschler, and Matthias Schieker, “Quantification of Fluorescence Intensity of Labeled Human Mesenchymal Stem Cells and Cell Counting of Unlabeled Cells in Phase-Contrast Imaging: An Op,” Tissue Engineering Part C-Methods, vol. 16, no. 6, pp. 1277–1285, 2010. View at Publisher · View at Google Scholar
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  • Dan Dominik Bruellmann, Andreas Pabst, Karl M. Lehmann, Thomas Ziebart, Marc O. Klein, and Bernd d'Hoedt, “Counting touching cell nuclei using fast ellipse detection to assess in vitro cell characteristics: a feasibility study,” Clinical Oral Investigations, vol. 16, no. 1, pp. 33–38, 2012. View at Publisher · View at Google Scholar
  • John H. Phan, Chang F. Quo, Chihwen Cheng, and May Dongmei Wang, “Multiscale integration of -omic, imaging, and clinical data in biomedical informatics,” IEEE Reviews in Biomedical Engineering, vol. 5, pp. 74–87, 2012. View at Publisher · View at Google Scholar
  • S. Kothari, J. H. Phan, T. H. Stokes, and M. D. Wang, “Pathology imaging informatics for quantitative analysis of whole-slide images,” Journal of the American Medical Informatics Association, vol. 20, no. 6, pp. 1099–1108, 2013. View at Publisher · View at Google Scholar
  • Yan Xu, Weiying Wu, Eric I-Chao Chang, Danny Chen, Jian Mu, Peter P. Lee, Kim R.M. Blenman, and Zhuowen Tu, “A two-layer structure prediction framework for microscopy cell detection,” Computerized Medical Imaging and Graphics, vol. 41, pp. 29–36, 2015. View at Publisher · View at Google Scholar
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