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Cellular Oncology
Volume 26, Issue 3, Pages 143-150

Interobserver Reproducibility of Dna-Image-Cytometry in Ascus or Higher Cervical Cytology

Vu Quoc Huy Nguyen,1,2 Hans Jürgen Grote,2 Natalia Pomjanski,2 Kristiane Knops,2 and Alfred Böcking2

1Department of Obstetrics and Gynecology, Hue University Medical School and Hospital, 6 Ngo Quyen Street, 47000 Hue, Vietnam
2Institute of Cytopathology, Heinrich‐Heine University Düsseldorf, Moorenstrasse 5, D‐40225 Düsseldorf, Germany

Received 17 August 2003; Accepted 19 April 2004

Copyright © 2004 Hindawi Publishing Corporation and the authors. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In the present study, the aim has been to investigate the interobserver reproducibility of DNA‐image‐cytometry (DNA‐ICM) applied to routine Pap smears classified as Atypical Squamous Cells of Undetermined Significance (ASCUS) or higher lesions (ASCUS+). 202 Pap smears diagnosed as ASCUS or higher were included in the study. After cytological assessment, smears underwent restaining according to Feulgen. First measurements were performed as routine workup. The second measurements were blinded to the result of the first and consecutively performed. DNA‐ICM met the consensus statements of the European Society of Analytical Cellular Pathology (ESACP). Interobserver agreement was assessed by calculating Kappa statistics. The diagnosis of DNA‐aneuploidy in the first measurements was confirmed in all cases. Second measurement detected 12 additional cases with aneuploidy. Nine out of these cases were classified as aneuploidy by detection of 9c Exceeding Events (9cEE). In three cases stemline‐aneuploidy was disclosed. The overall proportion of observed agreement was 94.1%, κ=0.87, 95% CI=0.74–0.99. Our study shows a good interobserver reproducibility of DNA‐ICM performed on cervical smears with ASCUS or higher lesions. DNA‐ICM thus represents a highly reproducible diagnostic procedure.