Figure 6: Molecular detection of T. gondii by nested-PCR of a B1 gene sequence. (a) First round PCR and (b) nested-PCR. Line 1: DNA from PF- and PE-sections of the frontal lobe SOL; line 2: DNA from PF- and PE-liver sections from a T. gondii experimentally infected mice; line 3: distilled water as a negative control; line 4: DNA from tachyzoites of T. gondii (RH strain); and line 5: 100 bp DNA ladder as molecular marker.