An Efficient Protocol for Deriving Liver Stem Cells from Neonatal Mice: Validating Its Differentiation Potential

Figure 4

Differentiation potential of mouse liver-derived stem cells. (a, c) Uninduced LDSCs with their normal morphology stained negative for Oil Red O stain (a) and alizarin red (c). (b) Adipogenesis induced LDSCs were stained with Oil Red O stain showing positive for oil droplets after 4 weeks of induction. (d) Osteogenesis induced LDSCs were stained with alizarin red showing mineralized nodule after 4 weeks of induction. (e) Phase contrast image of hepatogenesis induced LDSCs showing typical polygonal shape from fibroblast shape. (f) Hepatogenesis was shown positive for PAS staining (glycogen storage). (g) Albumin expression in hepatocyte induced LdSCs showed by ICC. Scale bars represent 100 μm (a)–(g).

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